pK7WGF2植物绿色荧光表达载体gateway质粒N端GFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pK7WGF2植物绿色荧光表达载体gateway质粒N端GFP

N-terminus GFP-CHX Vector (pK7WGF2)
Features of pK7WGF2


Feature Position Benefit

T35S Terminator Bases 18-243 Terminator of 35S

attR2 recombination site Bases 253-377 (Complementary) Allows recombinational cloning of the gene of interest from an entry clone

ccdB gene Bases 418-723 (Complementary) Allows negative selection of expression clones

Chloramphenicol resistance gene, CmR Bases 1065-1724 (Complementary) Allows counterselection of expression clones

attR1 recombination site Bases 1833-1957 (Complementary) Allows recombinational cloning of the gene of interest from an entry clone

Egfp Bases 1964-2680 (Complementary) enhanced green-fluorescent protein

p35S Bases 2702-3729 (Complementary) 35S promoter plus a part of omega leader

RB Bases 3749-3948

Sm/SpR Bases 8852-10101 (Complementary)

LB Bases 10107-10439 Allows selection of transformants in E.coli

Kan Bases 10443-11839 (Complementary)

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