pK7FWG2植物绿色荧光表达载体gateway质粒C端GFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pK7FWG2植物绿色荧光表达载体gateway质粒C端GFP

C-terminus CHX-GFP Vector (pK7FWG2)
Features of pK7FWG2


Feature Position Benefit

T35S Terminator Bases 18-243 Terminator of 35S

Egfp Bases 247-966 (Complementary) enhanced green-fluorescent protein

attR2 recombination site Bases 974-1098 (Complementary) Allows recombinational cloning of the gene of interest from an entry clone

ccdB gene Bases 1139-1444 (Complementary) Allows negative selection of expression clones

Chloramphenicol resistance gene, CmR Bases 1786-2445 (Complementary) Allows counterselection of expression clones

attR1 recombination site Bases 2554-2678 (Complementary) Allows recombinational cloning of the gene of interest from an entry clone

p35S Bases 2706-3733 (Complementary) 35S promoter plus a part of omega leader

RB Bases 3753-3952

Sm/SpR Bases 8856-10105 (Complementary)

LB Bases 10111-10443 Allows selection of transformants in E.coli

Kan Bases 10447-11843 (Complementary)


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