BV-173 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BV-173 cell line细胞株


Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the peripheral blood of a 45-year-old man with chronic myeloid leukemia (CML) in blast crisis in 1980; contains the t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Reference(s):
14479, 14488, 14840, 18187
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
round to elongated, single cells in suspension ; image; image; image; image; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; cells may grow slowly; split saturated culture 1:2 to 1:3 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at 2-3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 +, CD13 +, CD19 +, CD37 -, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed originally as human with IEF of G6PD, MDH, NP
Cytogenetics:
human hyperdiploid karyotype - 47(46-48)<2n>X/XY, -9, +22, +mar, add(1)(q42), add(8)(p23), t(9;22)(q34;q11), der(22)t(9;22)(q34;q11), der(?)t(9;?)(?p11;?) - resembles published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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