HCC2218 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HCC2218 cell line细胞株

Organism Homo sapiens, human
Tissue mammary gland; breast/duct
Cell Type Epithelial,lymphoblast
Product Format frozen
Morphology epithelial
Culture Properties suspension, The cells grow as floating aggregates of large cell clusters.
Biosafety Level 1
Disease TNM stage IIIA, grade 3,primary ductal carcinoma
Age 38 years adult
Gender female
Ethnicity Caucasian, White
Storage Conditions liquid nitrogen vapor phase
Karyotype polyploid
Images
Derivation
HCC2218 was derived from a female whose family members included several members with cancer; four lung cancer patients (two of them, non-smokers), two breast cancer patients (one of them also had lung cancer) and one colon cancer patient.
The HCC2218 cell line was initiated on April 10, 1996, and took 6 months to establish.
HCC2218 is a poorly differentiated cell line derived from the primary tumor of an invasive ductal carcinoma with metastases in 42/43 lymph nodes.
Clinical Data
38 years
Caucasian, White
female

Oncogene her2/neu +, p53 +
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Cellular Products
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Comments

The cells are highly positive for expression of Her2/neu, and are also positive for expression of p53.

HCC2218 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19.

The cells are negative for expression of estrogen receptor (ER -).

An EBV transformed lymphoblastoid cell line [HCC2218 BL] from the same patient is available as ATCC CRL-2363.

The cells are highly positive for expression of Her2/neu, and are also positive for expression of p53.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C

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