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pRS313酵母表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥19865
  • 货  号:pRS313酵母表达载体质粒
  • 产  地:北京
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pRS313酵母表达载体质粒

One of a series of pBluescript­based centromere vectors differing in theyeast selectable marker gene. A YC­type centromere vector permitting visual detection of recombinants andproduction of ssDNA in E. coli. It contains promoters for in vitro RNA synthesis, priming sites useful forsequencing, and encodes the lacZ alpha (lacZ​) peptide. pRSS56, constructed by ligating a PvuI fragment (bp498­2412) of pBluescript KS+ to a PvuI fragment (bp 2850­730) of pBS(+), contains the KS MCS frompBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment (1.184kb) containing the HIS3 gene was inserted into the NdeI site and a cassette contiaing CEN6 and the ARSassociated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted.

Size (kb): 4.967
Vector: pRS313 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRSS56 [pBluescript KS+, pBS(+)]
Marker(s):ampR,HIS3
Construct size (kb): 4.967
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: SacI...KpnI
centromere: CEN6

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