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pRS313酵母表达载体质粒
One of a series of pBluescriptbased centromere vectors differing in theyeast selectable marker gene. A YCtype centromere vector permitting visual detection of recombinants andproduction of ssDNA in E. coli. It contains promoters for in vitro RNA synthesis, priming sites useful forsequencing, and encodes the lacZ alpha (lacZ) peptide. pRSS56, constructed by ligating a PvuI fragment (bp4982412) of pBluescript KS+ to a PvuI fragment (bp 2850730) of pBS(+), contains the KS MCS frompBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment (1.184kb) containing the HIS3 gene was inserted into the NdeI site and a cassette contiaing CEN6 and the ARSassociated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted.Size (kb): 4.967Vector: pRS313 (phagemid)Promoters: Promoter for in vitro transcription T7Construction: pRSS56 [pBluescript KS+, pBS(+)]Marker(s):ampR,HIS3Construct size (kb): 4.967Features: insert detection: lacZ'marker(s): HIS3marker(s): ampRpromoter: lacpromoter for in vitro transcription: T3promoter for in vitro transcription: T7replicon: ARSH4replicon: f1replicon: pMB1MCS: SacI...KpnIcentromere: CEN6
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
One of a series of pBluescriptbased centromere vectors differing in theyeast selectable marker gene. A YCtype centromere vector permitting visual detection of recombinants andproduction of ssDNA in E. coli. It contains promoters for in vitro RNA synthesis, priming sites useful forsequencing, and encodes the lacZ alpha (lacZ) peptide. pRSS56, constructed by ligating a PvuI fragment (bp4982412) of pBluescript KS+ to a PvuI fragment (bp 2850730) of pBS(+), contains the KS MCS frompBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment (1.184kb) containing the HIS3 gene was inserted into the NdeI site and a cassette contiaing CEN6 and the ARSassociated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted.Size (kb): 4.967Vector: pRS313 (phagemid)Promoters: Promoter for in vitro transcription T7Construction: pRSS56 [pBluescript KS+, pBS(+)]Marker(s):ampR,HIS3Construct size (kb): 4.967Features: insert detection: lacZ'marker(s): HIS3marker(s): ampRpromoter: lacpromoter for in vitro transcription: T3promoter for in vitro transcription: T7replicon: ARSH4replicon: f1replicon: pMB1MCS: SacI...KpnIcentromere: CEN6
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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