pTriplEx原核表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pTriplEx原核表达载体

λTriplEx is a phagemid vector with cre-lox-mediated subcloningVery high-titer libraries (i.e., > 1 x 106 independent clones) can be constructedin λ vectors because of the high efficiency of packaging and transduction ofrecombinant λ phage into E. coli (Sambrook et al., 1989). In λTriplEx phagemidvectors, the cloning sites are located within a plasmid that is embedded in a λphage genome and flanked by loxP sites at the λ junctions (Figure 2). Transduc-ing a λTriplEx lysate into an appropriate E. coli strain such as BM25.8 promotesCre recombinase-mediated release and circularization of pTriplEx at the loxPsites (Elledge et al., 1991). Because helper phage is not required, Cre-lox-mediated phagemid conversion is an easy and reliable way to obtain a plasmidvector containing a desired cDNA clone. pTriplEx contains elements thatfacilitate its selection and autonomous replication in E. coli

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