BaF3_EGFR-T263P-G719S_Cell_Line激酶靶点稳转细胞株-BioVector NTCC典型培养物保藏中心

BaF3_EGFR-T263P-G719S_Cell_Line激酶靶点稳转细胞株-BioVector NTCC典型培养物保藏中心

Cell line name:Ba/F3 EGFRT263P/G719SCell LineGene ID/Accession #: NM_005228.3Host cell lineMouse Ba/F3 cell line Cell type: Pro-B cellsDescription: Stable Ba/F3 clone expressing exogenousEGFR genebearingT263Pand G719Sdoublemutations.Quantity:One vial of frozen cells (5X106per vial)Stability:Stable in culture over a minimum of 10 passagesApplication: Drug screening and biological assaysFreeze medium:70% RMPI-1640 + 20% FBS + 10% DMSOPropagation medium: RPMI 1640 + 10% FBSSelection marker: PuromycinMorphology: Mostly single, round (some polymorph) cells in suspensionSubculture: Split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105cells/mlIncubation: 37 °C with 5% CO2Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSODoubling time: Approximately 20 hoursMycoplasma status:NegativeBiosafety level: 1 Storage:Liquid nitrogen immediately upon receivingII．BackgroundEGFR, the Epidermal growth factor receptor, is a cell-surface receptor tyrosine kinase, and activated by binding of its specific ligand, such as epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha), EGFR overexpression or overactivity have associated with a number of cancers, including the lung cancer and colon cancer. The identification of EGFR as adriver gene has led to the development of anticancer therapeutics agents, including Gefitinib, Erlotinib, Afatinib, Osimertinib (AZD9291) and cetuximab.Ba/F3cell, a murine interleukin-3 dependent pro-B cell line, is a popular system for exploring both kinases and their inhibitors, because some protein kinases can render the Ba/F3cellsto be depended on the activation of the kinases instead ofIL-3supplement, whiletheirinhibitorscan antagonizethekinase-dependent growth effects.III．Cell Line GenerationBa/F3 EGFRG719S/T263Pcell Line was generated usingretrovirus vectorexpressing human EGFR sequencebearingG719Sand T263Pdouble mutations

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