首页 » pTCV-lac粪肠球菌穿梭质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pTCV-lac粪肠球菌穿梭质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥49865
  • 货  号:pTCV-lac粪肠球菌穿梭质粒
  • 产  地:北京
点击询问我要采购
 竭诚为您服务!
BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

电话:+86-010-53513060. 工作QQ:1843439339 (微信同号)

邮件:Biovector@163.com

手机:18901268599

地址:北京

已注册
 

pTCV-lac粪肠球菌穿梭质粒

Schematic representations of plasmids pTCV-lac and pTCV-tet. (A) The components of the parental vector pTCV-lac are (i) the spoVG-lacZ translational gene fusion; (ii) the genes aphA3 and ermB, conferring resistance to kanamycin and erythromycin, respectively; (iii) the replication origins (oriR) of plasmids pACYC184 and pAMβ1; and (iv) the transfer origin (oriT) of the IncP plasmid RK2. Plasmid pTCV-tet was constructed by replacing the BamHI-PstI spoVG-lacZ-ermB fragment of pTCV-lac with a BamHI-PstI fragment containing the tet(M) gene of Tn916 devoid of its translational signals, including the first codon. The plasmid components are not drawn to scale. (B) Expression of the tet(M) reporter gene of pTCV-tet was obtained following cloning of EcoRI-BamHI fragments containing various tet(M) promoter regions to provide appropriate transcriptional and translational signals. MCS, multiple cloning site. (C) Expression of the spoVG-lacZ reporter gene of pTCV-lac was obtained following cloning of the same EcoRI-BamHI fragments as those for panel B to provide appropriate transcriptional signals. Note that in all the spoVG-lacZ fusions analyzed in this study, tet(M) translation ends prematurely at the underlined TAA stop codon and hence should not interfere with that of the downstream reporter gene.

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net

您正在向 biovector.net  发送关于产品 pTCV-lac粪肠球菌穿梭质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问

点击“立即发送”后,我们将在1个工作日内与您取得联系。