首页 » B16-F10-Luc2 cell line小鼠荧光稳转细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

B16-F10-Luc2 cell line小鼠荧光稳转细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥98685
  • 货  号:B16-F10-Luc2
  • 产  地:北京
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Organism Mus musculus, mouseTissue skinProduct Format frozen 1.0 mLMorphology mixture of spindle-shaped and epithelial-like cellsCulture Properties adherentBiosafety Level 2Strain C57BL/6JApplications Excellent signal/background ratio and stable Luciferase expression make this cell line ideal for in vivo bioluminescence imaging of xenograft animal model to study human cancer and monitor activity of anti-cancer drug. It also can be used in cell-based assays for cancer research.Tumorigenic Yes, tested in C57/BL6Comments This luciferase expressing cell line was derived from parental line CRL-6475 by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter. This cell line was established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and in vivo bioluminescence assays. The cells should be maintained in Blasticidin (10 µg/mL) containing medium in routine cell culture. It is recommended to remove Blasticidin prior to and during the experiment procedure when the cells are injected into animals in vivo, or co-cultured with other cell types in vitro.Note: This cell line produces melanin. This may cause the culture medium or cells to appear dark brown or black, especially when the cultures are approaching a high level of confluence.Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium, (DMEM). To make the complete growth medium, add the following components to the base medium:Fetal bovine serum (FBS) to a final concentration of 10%Blasticidin to a final concentration of 10 µg/mLSubculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks are recommended for subculturing this product.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Cultures can be established between 2 x 104 and 4 x 104 viable cells/cm2.Incubate cultures at 37°C.div>Interval: Maintain cultures at a cell concentration between 1 X 104 and 2.3 X 105 cell/cm2.Subcultivation Ratio: A subcultivation ratio of 1:10 is recommendedMedium Renewal: Every2 to 3 daysCryopreservation Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CCells per Vial ≥ 1.0 x 106Volume 1.0 mLSterility Tests Bacteria and yeast: No growthMycoplasma: No growthFunctional Tests Luciferase activity: signal to noise ≥ 1,000 RLUsIn Vitro Luminesence: 20,000 photons/cell/sec, subject to imaging and culturing conditionsPopulation Doubling Time approximately 12 hrsYear of Origin 2018

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