K6H6/B5 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

K6H6/B5 cell line细胞株

Organism human (B cell lymphoma); mouse (myeloma) Tissue lymph node Cell Type hybridoma: B lymphocyte; somatic cell hybrid Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Disease nodular lymphoma Applications The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells. Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin. Tested and found negative for ectromelia virus (mousepox). Derivation The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma. Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells. Comments The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma. Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells. Tested and found negative for ectromelia virus (mousepox). Complete Growth Medium The base medium for this cell line is RPMI-1640 Medium, . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Medium Renewal: Every 2 to 3 days Cryopreservation Complete growth medium described above supplemented with 5% (v/v) DMSO.  Culture Conditions Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% Name of Depositor R Levy Deposited As human (B cell lymphoma); mouse (myeloma) Passage History The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma. Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells. References Carroll WL, et al. Mouse X human heterohybridomas as fusion partners with human B cell tumors. J. Immunol. Methods 89: 61-72, 1986. PubMed: 3084658

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