大肠杆菌E.coli SW106 strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

大肠杆菌E.coli SW106 strain菌株

SW102: a modified DH10B strain containing the defectivelambda prophage;DH10B[lcl857(cro-bioA)<>tet]. In addition this strain contains afully functional gal operon, except for a deletion of galK. This feature allows forefficient BAC modification using galK positive/negative selection. Derived from thestrain DY380. This strain is tetracycline resistant (12.5 ug/ml).(Red capped vial) SW105: a modified DH10B strain containing the defective lambdaprophage and an arabinose-inducible flpe gene; DH10B[lcl857(cro-bioA)<>araCPBADflpe]. In addition this strain contains a fully functional gal operon, except for adeletion of galK. This feature allows for efficient BAC modification using galKpositive/negative selection. Derived from the strain EL250. This strain carries noantibiotic resistance.(Green capped vial)) SW106: a modified DH10B strain containing the defectivelambda prophage and an arabinose-inducible cre gene; DH10B[lcl857(crobioA)<>araC- PBADcre]. In addition this strain contains a fully functional gal operon,except for a deletion of galK. This feature allows for efficient BAC modification usinggalK positive/negative selection. Derived from the strain EL350. This strain carries noantibiotic resistance.(Blue capped vial) positive contol: SW102 bacteria containing a BAC vector withthe galK expression cassette (cloned into the BamHI and HindIII sites ofpBeloBAC11).
pIGCN21: a vector containing the IRES-eGFPcre-FRT-kan-FRT cassette pELO4: a vector containing the Cam-sacB cassette pTamp: a vector containing an Amp cassette for replacing the loxP site inpBeloBAC11. Please note that this vector cannot be used toreplace either of the two loxP sites in the pBACe3.6 backbone. PL451: FRT-PGK-EM7-NeobpA-FRT-loxP PL452: loxP-PGK-EM7-NeobpA-loxP PL253: Modified MC1TK pgalK: a vector containing an em7-driven galK cassette for use in galKbased BAC modification using positive/negative selection


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