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YVH10 yeast strain酵母菌株
Saccharomyces cerevisiae Classification Fungi, Ascomycota, Saccharomycotina, Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomyces Strain Designations YVH10 Alternate State Candida robusta Diddens et Lodder Application Used in the yeast display method to isolate novel proteins based on various biological functions Biosafety Level 1 Genotype MATalpha PDI1::GAPDH-PDI1::LEU2 ura3- 52 trp1 leu2-delta200 his3-delta200 pep4::HIS3 prbd1.6R can1 GAL Preceptrol® no Comments YVH10 (Ura-, Trp-) is BJ5464 and is MAT-alpha. The other markers are: ura3-52 (a Ty element insertion with no detectable background reversion frequency), trp1 (an amber point mutation), leu2-delta200, his3-delta200, pep4:HIS3, prbd1.6R, can1, GAL. YVH10 has the yeast PDI gene integrated in tandem with the endogenous copy and is under the constitutive glyceraldehydes (GADPH) promoter with Leu2 as a selectable marker. Morphology On YM medium at 30°C for 2 days, colonies creamy white to dingy white, butyrous, smooth, slightly raised, margin entire. Cells globose to subglobose, hyaline, smooth, contain oil droplets, 4-6 X 3-5 µm. Pseudohyphae not observed. Medium YEPD Growth Conditions Temperature: 30°C Atmosphere: Typical aerobic
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TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Saccharomyces cerevisiae Classification Fungi, Ascomycota, Saccharomycotina, Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomyces Strain Designations YVH10 Alternate State Candida robusta Diddens et Lodder Application Used in the yeast display method to isolate novel proteins based on various biological functions Biosafety Level 1 Genotype MATalpha PDI1::GAPDH-PDI1::LEU2 ura3- 52 trp1 leu2-delta200 his3-delta200 pep4::HIS3 prbd1.6R can1 GAL Preceptrol® no Comments YVH10 (Ura-, Trp-) is BJ5464 and is MAT-alpha. The other markers are: ura3-52 (a Ty element insertion with no detectable background reversion frequency), trp1 (an amber point mutation), leu2-delta200, his3-delta200, pep4:HIS3, prbd1.6R, can1, GAL. YVH10 has the yeast PDI gene integrated in tandem with the endogenous copy and is under the constitutive glyceraldehydes (GADPH) promoter with Leu2 as a selectable marker. Morphology On YM medium at 30°C for 2 days, colonies creamy white to dingy white, butyrous, smooth, slightly raised, margin entire. Cells globose to subglobose, hyaline, smooth, contain oil droplets, 4-6 X 3-5 µm. Pseudohyphae not observed. Medium YEPD Growth Conditions Temperature: 30°C Atmosphere: Typical aerobic
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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