pScI_dCas9-CDA-UL plasmid细菌基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pScI_dCas9-CDA-UL plasmid细菌基因编辑质粒载体

Bacterial Target-AID UGI-LVA vector
Vector backbonepSC101Backbone size w/o insert (bp)9263Total vector size (bp)10930Vector typeBacterial ExpressionGROWTH IN BACTERIABacterial Resistance(s)Chloramphenicol low (12.5 ug/ml)Growth Temperature30°CGrowth Strain(s)Top10Growth instructionsAllow cells to recover for 2 hr in SOC medium. Transformed cells grow slow and colony formation takes 2 days. Do not pick larger colonies as escape cells may appear.Copy numberLow CopyGENE/INSERTGene/Insert namedCas9-PmCDA1-UGI-LVASpeciesSynthetic; Streptococcus pyogenesInsert Size (bp)7252MutationD10A and H840A for SpCas9GenBank IDOYN72315.1 ABO15149.1Promoterlambda ORTag / Fusion ProteinFlag

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