pER8GW plasmid植物雌激素诱导型双元表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pER8GW plasmid植物雌激素诱导型双元表达载体

Name    pER8GWType    plasmidTair Accession   Vector:6530631930Description    Gateway-compatible binary vector used to generate the TRANSPLANTA collection of lines
Schematic map of the T-DNA region of pER8GW vector. XVE: chimaeric XVE fusion gene, encoding the chimaeric transcription activator for the pLexA promoter, HPT: hygromycin phosphotransferase gene, pLexA: LexA operator fused to a minimal promoter of Cauliflower Mosaic Virus 35S gene, attR1 and attR2: Gateway recombination sites, CmR: Chloramphenicol resistance gene, ccdB: suicide marker for bacterial contraselection, T: RUBISCO rbcsS3A polyA sequence; RB and LB: T-DNA left and right border sequences, respectively. cDNA: randomly inserted cDNA clone; A and B: positions of T-DNA specific PCR primers used for amplification of inserted cDNAs.

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