首页 » Fluorescent Protein Guide: Empty Backbones荧光表达空载体介绍-BioVector NTCC保藏中心

Fluorescent Protein Guide: Empty Backbones荧光表达空载体介绍-BioVector NTCC保藏中心

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Background

Fluorescent_Cells.jpg

Fluorescent proteins are genetically encoded tools that are used extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 (Prasher et al., Gene, 1992), and since then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set of colors.

BioVector has assembled a collection of empty plasmid backbones with different fluorescent tags for you to create fusion proteins with your gene of interest. These are organized By Color of the fluorescent protein and By Expression Species .

The content for this page was generated with the help of Erik Snapp .

Empty Backbones for Fluorescent Protein Fusions (Organized by Color)

For each table:

  • Excitation and Emission maximum are listed in nm.

  • Brightness is the product of exctinction coefficient and quantum yield divided by 1000.

  • pKa is the pH at which fluorescence intensity is 50% of its maximum value.

  • Maturation is the time for fluorescence to obtain half-maximal value.

  • Structure describes the monomeric or oligomeric state of the protein. Many FPs are monomers at low concentration but are prone to dimerization at high concentration, so this is noted here. Proteins that are derived from GFP and contain the A206K mutation are monomeric at all concentrations, so this is mutation is noted when present.

  • For more information on the properties of many of these proteins, see:

    • Cranfill et al., Nature Methods 2016 Jul;13(7):557-62.

    • FPbase: A database for users of fluorescent proteins created by Talley Lambert at Harvard Medical School

    • More References below


Jump to:

  • Blue/UV

  • Cyan

  • Green

  • Yellow

  • Orange

  • Red

  • Far-Red

  • Near-Infrared

  • Long Stokes Shift

  • Photoactivatable

  • Photoconvertible

  • Photoswitchable

  • Timers


Blue/UV

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
Sirius35542443.0
Prone to dimerization
  • Sirius-N1 - Mammalian Expression

SBFP238044613

Monomer (A206K)
  • pSBFP2-C1 - Mammalian Expression

Azurite383450145
Prone to dimerization
  • pLV-Azurite - Mammalian Lentiviral expression

  • pB18cmBFPAzurite - Bacterial Expression

mAzurite383450145
Monomer (A206K)
  • mAzurite-N1 - Mammalian Expression

  • mAzurite-C1 - Mammalian Expression

EBFP2383448174.5
Prone to dimerization
  • pBad-EBFP2 - Bacterial Expression

  • EBFP2-N1 - Mammalian Expression

  • EBFP2-C1 - Mammalian Expression

  • EBFP2-pBAD - Bacterial Expression

moxBFP38544817

Monomer (A206K)
  • moxBFP - Mammalian Expression

mKalama1385456165.5
Monomer (A206K)
  • pBad-mKalama1 - Bacterial Expression

mTagBFP2399456322.712 minProne to dimerization
  • mTagBFP2-pBAD - Bacterial Expression

Jump to Top

Cyan

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
Aquamarine420474233.3
Prone to dimerization
  • pPROEX Aqua - Bacterial Expression

  • pAquaN1 - Mammalian Expression

ECFP433475134.7
Prone to dimerization
  • pcDNA3-CFP - Mammalian Expression

  • pCAG-CFP - Mammalian Expression

Cerulean433475274.7
Monomer (A206K)
  • Cerulean - Mammalian Expression

  • pCS2+8CCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin

  • pCS2+8NCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin

  • mCerulean N1 - Mammalian Expression

  • mCerulean C1 - Mammalian Expression

mCerulean3433475323.2
Monomer (A206K)
  • mCerulean3-N1 - Mammalian Expression

  • mCerulean3-C1 - Mammalian Expression

moxCerulean3434474363.2
Monomer (A206K)
  • moxCerulean3 - Mammalian Expression

SCFP3A433474174.5
Monomer (A206K)
  • pSCFP3A-C1 - Mammalian Expression

mTurquoise2434474283.1
Monomer (A206K)
  • pmTurquoise2-C1 - Mammalian Expression

  • pmTurquoise2-N1 - Mammalian Expression

CyPet435477185
Monomer
  • pCEP4CyPet-MAMM - Mammalian Expression

  • pCyPet-C1 - Mammalian Expression

  • pCyPet-His - Bacterial Expression

  • CYPet-N1 - Mammalian Expression

  • CYPet-C1 - Mammalian Expression

AmCyan145348611

Tetramer
  • AmCyan1-N1 - Mammalian Expression

MiCy (Midoriishi-Cyan)472495256.6
Dimer
  • MiCy-N1 - Mammalian Expression

  • MiCy-C1 - Mammalian Expression

  • MiCy-pBAD - Bacterial Expression

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Green

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
iLOV447497


Monomer
  • pEiLOV-N1 - Mammalian Expression

  • pGEX iLOV - Bacterial Expression

  • iLOV-N1 - Mammalian Expression

  • iLOV-C1 - Mammalian Expression

AcGFP147550528

Prone to dimerization
  • AcGFP1-N1 - Mammalian Expression

  • AcGFP1-C1 - Mammalian Expression

sfGFP48550754

Prone to dimerization
  • sfGFP-N1 - Mammalian Expression

  • sfGFP-C1 - Mammalian Expression

  • sfGFP-pBAD - Bacterial Expression

moxGFP48651050

Monomer (A206K)
  • moxGFP - Mammalian Expression

mEmerald487509396
Monomer (A206K)
  • mEmerald-N1 - Mammalian Expression

  • mEmerald-C1 - Mammalian Expression

  • mEmerald-pBAD - Bacterial Expression

EGFP488507346
Prone to dimerization
  • pcDNA3-EGFP - Mammalian Expression

  • pCAG-GFP - Mammalian Expression

  • pLV-eGFP - Mammalian Lentiviral Expression

  • Ac5-STABLE1-neo - Insect Expression

  • pCS2+8CeGFP - Zebrafish/Xenopus/C.elegans/Sea urchin

  • pCS2+8NeGFP - Zebrafish/Xenopus/C.elegans/Sea urchin

  • pKT0128 - Yeast Expression

  • EGFP-pBAD - Bacterial Expression

mEGFP488507346
Monomer (A206K)
  • pmEGFP-1 - Mammalian Expression

  • mEGFP-N1 - Mammalian Expression

  • mEGFP-C1 - Mammalian Expression

  • mEGFP-pBAD - Bacterial Expression

mAzamiGreen492505415.8
Monomer
  • mAzami Green-N1 (mAG) - Mammalian Expression

  • mAzami Green-C1 (mAG) - Mammalian Expression

cfSGFP2493517345.4
Monomer (A206K)
  • cfSGFP2-N - Mammalian Expression (cysteine-free SGFP2)

ZsGreen49350539

Tetramer
  • pHIV-Zsgreen - Mammalian Expression (this is a bicistronic vector rather than a fusion protein vector)

  • ZsGreen1-N1 - Mammalian Expression

SGFP249551230

Monomer (A206K)
  • pSGFP2-C1 - Mammalian Expression

Clover505515846.130 minProne to dimerization
  • pcDNA3-Clover - Mammalian Expression

  • pFA6a-link-yoClover-Kan - Yeast Expression

mClover2505515


Monomer (A206K)
  • mClover2-N1 - Mammalian Expression

  • mClover2-C1 - Mammalian Expression

mClover3506518856.5
Monomer (A206K)
  • pNCS-mClover3 - Bacterial Expression

mNeonGreen506517935.710 minMonomer
  • pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression

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Yellow

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
EYFP513527516.9
Prone to dimerization
  • pcDNA3-YFP - Mammalian Expression

Topaz51452757

Prone to dimerization
  • Topaz-N1 - Mammalian Expression

  • Topaz-C1 - Mammalian Expression

  • Topaz-pBAD - Bacterial Expression

mTopaz514527


Monomer (A206K)
  • mTopaz-N1 - Mammalian Expression

  • mTopaz-C1 - Mammalian Expression

  • mTopaz-pBAD - Bacterial Expression

mVenus515528536
Monomer (A206K)
  • mVenus N1 - Mammalian Expression

  • mVenus C1 - Mammalian Expression

  • pKT0090 - Yeast Expression

  • mVenus-pBAD - Bacterial Expression

moxVenus51452644

Monomer (A206K)
  • moxVenus - Mammalian Expression

SYFP2515527696
Monomer (A206K)
  • pSYFP2-C1 - Mammalian Expression

mGold515530685.9
Monomer
  • pJL-mGold - Yeast Expression

  • pCaggs-mGold - Mammalian Expression

mCitrine516529595.7
Monomer (A206K)
  • pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression

  • pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin

  • pCS2+8NmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin

  • pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression

  • mCitrine-N1 - Mammalian Expression

  • mCitrine-C1 - Mammalian Expression

  • mCitrine-pBAD - Bacterial Expression

YPet517530805.6
Prone to dimerization
  • pCEP4YPet-MAMM - Mammalian Expression

  • pYPet-His - Bacterial Expression

  • YPet-N1 - Mammalian Expression

  • YPet-C1 - Mammalian Expression

  • YPet-pBAD - Bacterial Expression

ZsYellow15295398

Tetramer
  • ZsYellow1-N1 - Mammalian Expression

mPapaya1530541366.8
Monomer
  • mPapaya1-N1 - Mammalian Expression

  • mPapaya1-C1 - Mammalian Expression

mCyRFP1528594185.6
Monomer
  • pNCS-mCyRFP1 - Bacterial Expression

  • mCyRFP1-C1 - Mammalian Expression

Jump to Top

Orange

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
mKusabira-Orange (mKO)5485593154.5 hrMonomer
  • pQC mKorange IX - Mammalian Expression

  • mKusabira Orange-C1 (mKO) - Mammalian Expression

  • mKO-pBAD - Bacterial Expression

mOrange548562496.52.5 hrMonomer
  • pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression

  • pCS2+8CmOrange - Zebrafish/Xenopus/C.elegans/Sea urchin

  • pCS2+8NmOrange - Zebrafish/Xenopus/C.elegans/Sea urchin

  • mOrange-N1 - Mammalian Expression

  • mOrange-C1 - Mammalian Expression

  • mOrange-pBAD - Bacterial Expression

mOrange2549565356.54.5 hrMonomer
  • mOrange2-N1 - Mammalian Expression

  • mOrange2-C1 - Mammalian Expression

  • mOrange2-pBAD - Bacterial Expression

mKO2551565405.5
Monomer
  • mKO2-N1 - Mammalian Expression

  • mKO2-C1 - Mammalian Expression

  • mKO2-pBAD - Bacterial Expression

TurboRFP553574624.4
Dimer
  • TurboRFP-pBAD - Bacterial Expression

tdTomato554581954.71 hrTandem-dimer
  • pCSCMV:tdTomato - Mammalian Expression

  • pFA6a-link-tdTomato-SpHis5 - Yeast Expression

  • tdTomato-N1 - Mammalian Expression

  • tdTomato-C1 - Mammalian Expression

  • tdTomato-pBAD - Bacterial Expression

Jump to Top

Red

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
mScarlet-H551592154.84.4 hrMonomer
  • pmScarlet-H_C1 - Mammalian Expression

mNectarine558578266.930 minMonomer
  • pBAD-mNectarine - Bacterial Expression

mRuby2559600435.3150 minProne to dimerization
  • pcDNA3-mRuby2 - Mammalian Expression

  • pFA6a-link-yomRuby2-SpHis5 - Yeast Expression

  • mRuby2-N1 - Mammalian Expression

  • mRuby2-C1 - Mammalian Expression

eqFP61155961135

Tetramer
  • eqFP611-N1 - Mammalian Expression

DsRed256358224

Tetramer
  • pCAG-DsRed2 - Mammalian Expression

  • DsRed2-N1 - Mammalian Expression

  • DsRed2-C1 - Mammalian Expression

  • DsRed2-pBAD - Bacterial Expression

mApple568592376.530 minMonomer
  • mApple-N1 - Mammalian Expression

  • mApple-C1 - Mammalian Expression

  • mApple-pBAD - Bacterial Expression

mScarlet569594715.32.9 hrMonomer
  • pmScarlet_C1 - Mammalian Expression

  • pCS2+mScarlet-C Cloning Vector - Mammalian Expression

mScarlet-I569593575.40.6 hrMonomer
  • pmScarlet-i_C1 - Mammalian Expression

mStrawberry574596264.550 minMonomer
  • mStrawberry-N1 - Mammalian Expression

FusionRed58060818

Monomer
  • FusionRed-pBAD - Bacterial Expression

mRFP1584607134.51 hrMonomer
  • pcDNA3-mRFP - Mammalian Expression

  • pMXs-mRFP1 - Mammalian Retroviral Expression

mCherry587610164.515 minMonomer
  • pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression

  • pLV-mCherry - Mammalian Lentiviral Expression

  • pCS2+8CmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin

  • pCS2+8NmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin

  • pET mCherry LIC cloning vector (u-mCherry) - Bacterial Expression

  • pFA6a-link-yomCherry-SpHis5 - Yeast Expression

mCherry2587610


Monomer
  • mCherry2-N1 - Mammalian Expression

  • mCherry2-C1 - Mammalian Expression

Jump to Top

Far-Red

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
mCrimson3588615-

Monomer
  • mCrimson3-N1 - Mammalian Expression

  • mCrimson3-C1 - Mammalian Expression

HcRed15886180.3

Dimer
  • pCAG-HcRed - Mammalian Expression

  • HcRed1-N1 - Mammalian Expression

  • HcRed1-C1 - Mammalian Expression

dKatushka588635225.5
Dimer
  • dKatushka-pBAD - Bacterial Expression

mKate1.358863525

Monomer
  • mKate1.31-pBAD - Bacterial Expression

mPlum59064944.5100 minMonomer
  • pQC mPlum IX - Mammalian Expression

  • mPlum-N1 - Mammalian Expression

  • mPlum-C1 - Mammalian Expression

  • mPlum-pBAD - Bacterial Expression

mRaspberry59862513
55 minMonomer
  • mRaspberry-N1 - Mammalian Expression

TagRFP67559867545
Prone to dimerization
  • pTagRFP675-N1 - Mammalian Expression

  • pBAD/HisD-TagRFP675 - Bacterial Expression

mNeptune600650135.435 minProne to dimerization
  • pcDNA3-mNeptune2-N - Mammalian Expression

mNeptune260065021

Prone to dimerization
  • mNeptune2-N1 - Mammalian Expression

  • mNeptune2-C1 - Mammalian Expression

mCarmine6036755.815.58
Monomer
  • mCarmine in pcDNA3 - Mammalian Expression

  • mCarmine in pRSETB - Bacterial Expression

mCardinal604659175.327 minProne to dimerization
  • mCardinal-N1 - Mammalian Expression

  • mCardinal-C1 - Mammalian Expression

  • mCardinal-pBAD - Bacterial Expression

mMaroon610652-

Monomer
  • mMaroon-N1 - Mammalian Expression

  • mMaroon-C1 - Mammalian Expression

TagRFP6576116570.345.327 minProne to dimerization
  • pTagRFP657-N1 - Mammalian Expression

  • pTagRFP657-C1 - Mammalian Expression

  • pFA6a-link-yoTagRFP657-Kan - Yeast Expression

smURFP + BV64267032
39 minProne to dimerization
  • smURFP + BV - Mammalian Expression

Jump to Top

Near-Infrared

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
miRFP670642670124.5
Monomer
  • pmiRFP670-N1 - Mammalian Expression

  • pBAD/His-miRFP670 - Bacterial Expression

iRFP670643670134~5 hrDimer
  • piRFP670-N1 - Mammalian Expression

  • pBAD/HisB-iRFP670 - Bacterial Expression

iRFP682663682104.5~5hrDimer
  • piRFP682-N1 - Mammalian Expression

  • pBAD/HisB-iRFP682 - Bacterial Expression

miRFP70367370384.5
Monomer
  • pmiRFP703-N1 - Mammalian Expression

  • pBAD/His-miRFP703 - Bacterial Expression

iRFP70267370284.5~5hrDimer
  • piRFP702-N1 - Mammalian Expression

  • pBAD/HisB-iRFP702 - Bacterial Expression

miRFP70968370944.5
Monomer
  • pmiRFP709-N1 - Mammalian Expression

  • pBAD/His-miRFP709 - Bacterial Expression

mIFP68370473.5
Monomer
  • mIFP-N1 - Mammalian Expression

  • mIFP24-C1 - Mammalian Expression

IFP2.06847087

Prone to dimerization
  • IFP2.0-N1 - Mammalian Expression

  • IFP2.0-C1 - Mammalian Expression

iRFP (aka iRFP713)69071364.52.8 hrDimer
  • piRFP - Mammalian Expression

  • pBAD/His-B-iRFP - Bacterial Expression

iSplit6907135

Dimer
  • pPAS-E - Mammalian Expression

  • pK-GAFm - Mammalian Expression

  • pWA PAS-E BAD K-GAFm - Bacterial Expression

iRFP72070272064.5~5hrDimer
  • piRFP720-N1 - Mammalian Expression

  • pBAD/HisB-iRFP720 - Bacterial Expression

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Long Stokes Shift

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
T-sapphire399511264.91.3 hrProne to dimerization
  • pFA6a-link-yETSapphire-CaUra3 - Yeast Expression

mT-sapphire399511


Monomer (A206K)
  • mT-Sapphire-N1 - Mammalian Expression

  • mT-Sapphire-C1 - Mammalian Expression

  • mT-Sapphire-pBAD - Bacterial Expression

mAmetrine4065262660.8 hrMonomer (A206K)
  • pBad-mAmetrine - Bacterial Expression

  • mAmetrine-N1 - Mammalian Expression

  • mAmetrine-C1 - Mammalian Expression

LSSmOrange437572235.72.3 hrMonomer
  • pLSSmOrange-C1 - Mammalian Expression

  • pLSSmOrange-N1 - Mammalian Expression

  • pBAD-LSSmOrange - Bacterial Expression

mKeima Red44062036.54.4 hrMonomer
  • pFA6a-link-yomKeima-CaURA3 - Yeast Expression

  • mKeima-Red-N1 - Mammalian Expression

  • mKeima-Red-C1 - Mammalian Expression

dKeima Red440616226.5
Dimer
  • dKeima-Red-N1 - Mammalian Expression

LSSmKate146362433.21.7 hrMonomer
  • pLSSmKate1-N1 - Mammalian Expression

  • pLSSmKate1-C1 - Mammalian Expression

  • pBAD/HisD-LSSmKate1 - Bacterial Expression

LSSmKate246060542.72.5 hrMonomer
  • pLSSmKate2-N1 - Mammalian Expression

  • pBAD/HisD-LSSmKate2 - Bacterial Expression

  • LSSmKate2-C1 - Mammalian Expression

Jump to Top

Photoactivatable (e.g. off to on)

Excitation and Emission wavelengths after activation.

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
Phamret45851714

Monomer
  • Phamret-N1 - Mammalian Expression

  • Phamret-C1 - Mammalian Expression

PA-sfGFP485511


Prone to dimerization
  • PA-sfGFP-N1 - Mammalian Expression

  • PA-sfGFP-C1 - Mammalian Expression

mPA-Emerald487509-

Monomer (A206K)
  • mPA-Emerald-N1 - Mammalian Expression

  • mPA-Emerald-C1 - Mammalian Expression

PA-GFP50451714

Monomer (A206K)
  • pPAGFP-N1 - Mammalian Expression

  • pPAGFP-C1 - Mammalian Expression

  • pRSETA-PAGFP - Bacterial Expression

  • tandem PA-GFP-KanR - Yeast Expression

  • pFA6a-PA-GFP-kanMX6 - Yeast Expression

  • pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression

  • mPA-GFP-N1 - Mammalian Expression

  • mPA-GFP-C1 - Mammalian Expression

PATagRFP562595255.375 minProne to dimerization
  • pBAD/HisD-PATagRFP - Bacterial Expression

PAmCherry156459586.323 minMonomer
  • pPAmCherry1-N1 - Mammalian Expression

  • pPAmCherry1-C1 - Mammalian Expression

  • pBAD/HisB-PAmCherry1 - Bacterial Expression

PAmCherry2570596136.218 minMonomer
  • pPAmCherry2-C1 - Mammalian Expression

PAmCherry357059656.225 minMonomer
  • pPAmCherry3-C1 - Mammalian Expression

PAmKate56862855.619 minMonomer
  • pPAmKate-N1 - Mammalian Expression

  • pBAD/HisB-PAmKate - Bacterial Expression

PAiRFP16907173

Dimer
  • pPAiRFP1-N1 - Mammalian Expression

  • pBAD/HisB-PAiRFP1 - Bacterial Expression

PAiRFP26927193

Dimer
  • pPAiRFP2-N1 - Mammalian Expression

  • pBAD/HisB-PAiRFP2 - Bacterial Expression

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Photoconvertible (e.g. green to red)

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
Dendra2490 / 553507 / 57323/196.6/6.9
Monomer
  • pEG545 - Worm Expression

mEos2506 / 571516 / 58147 / 305.6/6.4
Prone to oligomerization
  • pRSETa mEos2 - Bacterial Expression

  • pFA6a-link-yomEos2-CaURA3 - Yeast Expression

mEos3.2507 / 572516 / 58053 / 185.4/5.820 minMonomer
  • mEos3.2-N1 - Mammalian Expression

  • mEos3.2-C1 - Mammalian Expression

mEos4a505 / 569516 / 581


Prone to oligomerization
  • mEos4a-N1 - Mammalian Expression

  • mEos4a-C1 - Mammalian Expression

mEos4b505 / 569516 / 581


Monomer
  • mEos4b-N1 - Mammalian Expression

  • mEos4b-C1 - Mammalian Expression

dEos505 / 569516 / 58155 / 20

Dimer
  • dEos-N1 - Mammalian Expression

tdEos505 / 569516 / 58155 / 20

Tandem dimer
  • tdEos-N1 - Mammalian Expression

  • tdEos-C1 - Mammalian Expression

mKikGR507 / 583517 / 59334 / 186.6/5.2
Monomer
  • mKikGR-N1 - Mammalian Expression

  • mKikGR-C1 - Mammalian Expression

Kaede508 / 572518 / 58087 / 205.6/5.6
Tetramer
  • Kaede-N1 - Mammalian Expression

  • Kaede-C2 - Mammalian Expression

PSmOrange548 / 634565 / 66258 / 96.2/5.61.6 hrMonomer
  • pPSmOrange-N1 - Mammalian Expression

  • pPSmOrange-C1 - Mammalian Expression

  • pFA6a-link-yoPSmOrange-SpHis5 - Yeast Expression

PSmOrange2546 / 619561 / 65131 / 76.6/5.43.5 hrMonomer
  • pPSmOrange2-N1 - Mammalian Expression

  • pBAD-PSmOrange2 - Bacterial Expression

Jump to Top

Photoswitchable (e.g. off to on to off)

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
rsTagRFP440 / 567585 / 5850.02 / 4.06.643 minProne to dimerization
  • pBAD/HisD-rsTagRFP - Bacterial Expression

rsEmerald491508



  • rsEmerald-pBAD - Bacterial Expression

rsGFP1493510



  • rsGFP1-pBAD - Bacterial Expression

Dronpa350351819

Monomer
  • Dronpa3-N1 - Mammalian Expression

  • Dronpa3-C1 - Mammalian Expression

mGeos-M503 / 569514 / 581444.7
Prone to dimerization
  • mGeos-M-N1 - Mammalian Expression

  • mGeos-M-C1 - Mammalian Expression

Jump to Top

Fluorescent Timers

ProteinExcitation (nm)Emission (nm)BrightnesspKaMaturationStructurePlasmids
Fast-FT403(blue), 583(red)466(blue), 606(red)14.9(blue), 6.8(red)2.8/4.17.1 hr (red)Monomer
  • pFast-FT-N1 - Mammalian Expression

  • pTRE-Fast-FT - Mammalian Expression

  • pBAD/HisB-Fast-FT - Bacterial Expression

Medium-FT401(blue), 579(red)464(blue), 600(red)18.4(blue), 5.8(red)2.7/4.73.9 hr (red)Monomer
  • pMedium-FT-N1 - Mammalian Expression

  • pTRE-Medium-FT - Mammalian Expression

  • pBAD/HisB-Medium-FT - Bacterial Expression

Slow-FT402(blue), 583(red)465(blue), 604(red)11.7(blue), 4.2(red)2.6/4.628 hr (red)Monomer
  • pSlow-FT-N1 - Mammalian Expression

  • pTRE-Slow-FT - Mammalian Expression

  • pBAD/HisB-Slow-FT - Bacterial Expression



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