pKAS32 and pKAS46 plasmid等位基因交换质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥49865
- 货 号:pKAS32 and pKAS46
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
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手机:18901268599
地址:北京
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pKAS32 and pKAS46 plasmid等位基因交换质粒载体
two new allelic exchange vectors, pKAS32 and pKAS46. These vectors can be used for allelic exchange in a wide variety of bacterial species because their R6K origin of replication functions only in bacteria engineered to produce the replication protein pi. In addition, these vectors express the Escherichia coli rpsL gene, encoding ribosomal protein S12, which provides a positive selection for bacteria that have exchanged cloned plasmid sequences with the corresponding chromosomal sequences. In this report, we show that these vectors can be used to efficiently introduce point mutations and deletions into the chromosome of Vibrio cholerae.
Supplier来源:BioVector NTCC Inc.
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