u266，Karpas 707, W63, U-1996, U-266 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

u266，Karpas 707, W63, U-1996, U-266 cell line细胞株

Species:
human (Homo sapiens)
Cell type:
multiple myeloma
Origin:
established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968; cells were described in the literature to produce IgE lambda; possible fusion partner for hybridoma production; cells express mRNA for BCL2 gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Reference(s):
14805, 14486, 18187
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
round to polygonal, single or clustered cells in suspension, some loosely adherent; image; image; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-0.7 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; maintain at 0.2-1.0 x 106 cells/ml; cells grow partially (loosely) adherent and can be removed with a bent Pasteur pipette (trypsin is not necessary)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 55 hours
Harvest:
saturation density at 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays
Immunology:
CD3 -, CD5 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 (+), CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, smlambda -, cylambda +; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
originally confirmed as human with IEF of AST, MDH, NP and recently by STR fingerprinting
Cytogenetics:
human complex hypodiploid karyotype with 6.5% polyploidy - 44(40-46)<2n>XY, -8, -10, -13, -15, +2mar, t(1;11)(p33;q13), add(3)(q27), t(4;11)(q?21;q23), add(7)(q32), add(8)(q24), add(9)(q34), add(10)(p14), add(14)(p11), add(17)(p11), add(18)(p12), der(22)t(15;22)(q21;q13) - 11q13 breakpoint recurrent in multiple myeloma
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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