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Cell lines used in COVID-19 vaccine production疫苗生产用细胞株

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Cell lines used in COVID-19 vaccine production

A number of SARS-CoV-2 vaccines make use of cell lines to either grow an engineered adenovirus vector, an attenuated SARS-CoV-2 virus or a recombinant spike protein that will directly elicit an immune response.
  • HEK293 (CVCL_0045) for Gamaleya Gam-COVID-Vac (Sputnik V)

  • HEK293SF-3F6 (CVCL_4V95) for CanSino Biologics Convidecia (Ad5-nCOV)

  • K562/GM-CSF (CVCL_A8HT) for Sorento Therapeutics STI-6991 (in development)

  • PER.C6 TetR (CVCL_A7ME) for Janssen Ad26.COV2.S

  • Sf9 (CVCL_0549) for Novavax NVX-CoV2373

  • T-REx-293 (CVCL_D585) for AstraZeneca Vaxzevria (ChAdOx1-SARS-COV-2; AZD1222)

  • Vero (CVCL_0059) for Bharat Biotech Covaxin (BBV152) and for Sinovax CoronaVac (PiCoVacc)

For some vaccines in development, we are not sure of the cell line used for their production, however we have some clue that:
  • Sf900+ (CVCL_4U10) may be used for the candidate Sanofi vaccine VAT00002/VAT00008

It must be noted that, as described in a specific FAQ, vaccines do NOT contain cell lines.

For a non-controversial overview of the controversy on the use of human fetal cell lines for COVID-19 vaccine development see the following University of Nebraska Medical Center page.



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