pET-27b(+)原核表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

大肠杆菌表达质粒

The pET-27b(+) vector (Cat. No. 69863-3) carries an N-terminal pelB signal sequence for potential

periplasmic localization, plus optional C-terminal HSV•Tag® and His•Tag® sequences. This

vector differs from pET-25b(+) only by its selectable marker (kanamycin vs. ampicillin resistance).

Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322

convention, so the T7 expression region is reversed on the circular map. The cloning/expression

region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is

oriented so that infection with helper phage will produce virions containing single-stranded DNA

that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed

using the T7 terminator primer