pEZZ18大肠杆菌表达载体质粒-BioVector NTCC质粒载体菌种细胞基因保藏中心

LOCUS       pEZZ18    4591 bp    DNA    SYN    21-Nov-2013
DEFINITION  pEZZ18
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="pEZZ18"
                     /mol_type="other DNA"
     promoter        131..159
                     /label="AmpR_promoter"
     CDS             201..1061
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /note="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     gene            201..1061
                     /label="Ampicillin"
                     /gene="Ampicillin"
     rep_origin      1216..1835
                     /label="pBR322_origin"
     promoter        2144..2173
                     /label="lac_promoter"
     misc_feature    2187..2209
                     /label="M13_pUC_rev_primer"
     misc_feature    2208..2226
                     /label="M13_reverse_primer"
     CDS             2396..3157
                     /label="ORF frame 2"
                     /translation="MMTLQIHTGGINLKKKNIYSIRKLGVGIASVTLGTLLISGGVTP
                     AANAAQHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEA
                     KKLNDAQAPKVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEA
                     KKLNDAQAPKVDANSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLAAHP
                     PFASWRNSEEARTDRPSQQLRSLNGEWRFRCNGWR*"
     misc_feature    complement(2974..2990)
                     /label="M13_forward20_primer"
     misc_feature    complement(2983..3005)
                     /label="M13_pUC_fwd_primer"
     misc_feature    2983..3126
                     /label="lacZ_a"
     rep_origin      3448..3754
                     /label="f1_origin"
     misc_feature    4541..4563
                     /label="pGEX_3_primer"
ORIGIN
    1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 
   61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
  121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
  181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
  241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
  301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
  361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
  421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
  481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
  541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
  601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
  661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
  721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
  781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
  841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
  901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
  961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
 1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
 1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
 1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
 1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
 1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
 1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 
 1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
 1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
 1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
 1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
 1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
 1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
 1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
 1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
 1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
 1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
 1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
 2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
 2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 
 2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 
 2221 accatgatta cgaattagcg gccgctagcc gaaatagcgt gattttgcgg ttttaagcct 
 2281 tttacttcct gaataaatct ttcagcaaaa tatttatttt ataagttgta aaacttacct 
 2341 ttaaatttaa ttataaatat agattttagt attgcaatac ataattcgtt atattatgat 
 2401 gactttacaa atacatacag ggggtattaa tttgaaaaag aaaaacattt attcaattcg 
 2461 taaactaggt gtaggtattg catctgtaac tttaggtaca ttacttatat ctggtggcgt 
 2521 aacacctgct gcaaatgctg cgcaacacga tgaagccgta gacaacaaat tcaacaaaga 
 2581 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa 
 2641 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc 
 2701 taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca 
 2761 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt 
 2821 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa 
 2881 gctaaatgat gctcaggcgc cgaaagtaga cgcgaattcg agctcggtac ccggggatcc 
 2941 tctagagtcg acctgcaggc atgcaagctt ggcactggcc gtcgttttac aacgtcgtga 
 3001 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 
 3061 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 
 3121 tggcgaatgg cgattccgtt gcaatggctg gcggtaatat tgttctggat attaccagca 
 3181 aggccgatag tttgagttct tctactcagg caagtgatgt tattactaat caaagaagta 
 3241 ttgcgacaac ggttaatttg cgtgatggac agactctttt actcggtggc ctcactgatt 
 3301 ataaaaacac ttctcaggat tctggcgtac cgttcctgtc taaaatccct ttaatcggcc 
 3361 tcctgtttag ctcccgctct gattctaacg aggaaagcac gttatacgtg ctcgtcaaag 
 3421 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
 3481 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
 3541 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
 3601 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
 3661 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
 3721 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
 3781 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
 3841 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaat atttgcttat 
 3901 acaatcttcc tgtttttggg gcttttctga ttatcaaccg gggtacatat gattgacatg 
 3961 ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc aggcaatgac 
 4021 ctgatagcct ttgtagagac ctctcaaaaa tagctaccct ctccggcatg aatttatcag 
 4081 ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt tctcacccgt 
 4141 ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag ggttctaaaa 
 4201 atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag ggtcataatg 
 4261 tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat tttgctaatt 
 4321 ctttgccttg cctgtatgat ttattggatg ttggaattga tgcggtattt tctccttacg 
 4381 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 
 4441 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 
 4501 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 
 4561 aggttttcac cgtcatcacc gaaacgcgcg a
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