pME6032载体广宿主穿梭表达质粒含有Tac启动子-BioVector NTCC保藏中心

pME6032

特点：

广宿主载体

含有Tac强启动子

图谱Map：

序列Sequence

Shuttle vector pME6032, complete sequence

GenBank: DQ645594.1

FASTA Graphics

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Features

Sequence

LOCUS       DQ645594                9816 bp    DNA    circular SYN 27-JUN-2006

DEFINITION Shuttle vector pME6032, complete sequence.

ACCESSION   DQ645594

VERSION     DQ645594.1 GI:109452800

KEYWORDS   .

SOURCE     Shuttle vector pME6032

ORGANISM  Shuttle vector pME6032

         other sequences; artificial sequences; vectors.

REFERENCE  1  (bases 1 to 9816)

AUTHORS   Heeb,S., Itoh,Y., Nishijyo,T.,Schnider,U., Keel,C., Wade,J.,

         Walsh,U., O'Gara,F. and Haas,D.

TITLE     Small, stable shuttlevectors based on the minimal pVS1 replicon

         for use in gram-negative, plant-associated bacteria

JOURNAL   Mol. Plant MicrobeInteract. 13 (2), 232-237 (2000)

PUBMED   10659714

REFERENCE  2  (bases 1 to 9816)

AUTHORS   Heeb,S., Blumer,C. andHaas,D.

TITLE     Regulatory RNA asmediator in GacA/RsmA-dependent global control of

         exoproduct formation in Pseudomonas fluorescens CHA0

JOURNAL   J. Bacteriol. 184 (4),1046-1056 (2002)

PUBMED   11807065

REFERENCE  3  (bases 1 to 9816)

AUTHORS   Heeb,S.

TITLE     Direct Submission

JOURNAL   Submitted (19-MAY-2006)Institute of Infection, Immunity and

         Inflammation, University of Nottingham, Centre for Biomolecular

         Sciences, NottinghamNG7 2RD, United  Kingdom

FEATURES             Location/Qualifiers

  source          1..9816

                   /organism="Shuttlevector pME6032"

                   /mol_type="otherDNA"

                  /db_xref="taxon:390901"

  CDS             195..881

                   /note="ORF1; notrequired for stable maintenance"

                   /codon_start=1

                   /transl_table=11

                   /product="pVS1resolvase"

/protein_id="ABG33931.1"

                  /db_xref="GI:109452801"

                  /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITG

                   TRRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWA

                  DTTTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHAR

                  ELIDQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAA

                   AENERQEEQA"

CDS             878..1093

                   /note="ORF2; notrequired for stable maintenance"

                   /codon_start=1

                   /transl_table=11

                  /product="hypothetical protein"

/protein_id="ABG33932.1"

                  /db_xref="GI:109452802"

                  /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEIL

                  AGLSDAKLAAWPASLAAEETERRRLKR"

   CDS             1180..1809

                   /note="pVS1partitioning protein"

/codon_start=1

                   /transl_table=11

                   /product="StaA"

/protein_id="ABG33933.1"

                  /db_xref="GI:109452803"

                  /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA

                  RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL

                  IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE

                  LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

CDS             1830..2045

                   /note="pVS1partitioning protein"

                   /codon_start=1

                   /transl_table=11

                   /product="ParG"

/protein_id="ABG33934.1"

                   /db_xref="GI:109452804"

                  /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDH

                  TKLKMYAVRQGKTITELLSEYIAQLPE"

   CDS             2238..3311

                   /note="pVS1replication protein"

/codon_start=1

                   /transl_table=11

                   /product="RepA"

/protein_id="ABG33935.1"

                  /db_xref="GI:109452805"

                  /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGES

                  WQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFR

                  LSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKG

                  DKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTV

                  SEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIG

                  RFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEA

                  GQAGPILVMRYRNLIEGEASAGS"

   rep_origin      3377..3571

                   /note="pVS1 origin ofreplication"

rep_origin      4264..5089

                   /note="p15A origin ofreplication"

  oriT            5068..5089

                   /note="p15A origin oftransfer"

  terminator      5148..5414

                   /note="T4transcription terminator"

  misc_feature    5402..5421

                   /note="sequencingoligonucleotide P6032:

                  5'-CCCTCACTGATCCGCTAGTC-3'"

  misc_feature    5416..5473

                   /note="multiplecloning site"

  misc_feature   complement(5468..5542)

                   /note="tac promoter,5'UTR and RBS followed by EcoRI"

  RBS            complement(5478..5481)

  misc_feature   complement(5508..5527)

                   /note="sequencingoligonucleotide PTAC:

                  5'-CGGCTCGTATAATGTGTGGA-3'"

  misc_feature    complement(5509)

                   /note="start oftranscription"

  -10_signal     complement(5514..5520)

  -35_signal      complement(5537..5542)

  CDS             5977..6792

                   /note="tac promoterrepressor"

                   /codon_start=1

                   /transl_table=11

                   /product="LacIQrepressor"

/protein_id="ABG33936.1"

                  /db_xref="GI:109452806"

                  /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS

                  RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV

                   PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW

                  HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE

                  SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTTWTACCNSLRARR"

CDS             complement(7821..8498)

                   /note="RK2tetracycline resistance repressor protein"

                   /codon_start=1

                   /transl_table=11

                   /product="TetR"

                  /protein_id="ABG33937.1"

                  /db_xref="GI:109452807"

                  /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLG

                  VQQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAY

                  RDGARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAG

                   DSDAGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVE

                   GPRKGDD"

  CDS             8577..9776

                   /note="RK2tetracycline resistance protein"

                   /codon_start=1

                   /transl_table=11

                   /product="TetA"

                  /protein_id="ABG33938.1"

                  /db_xref="GI:109452808"

                  /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH

                   YGILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIG

                  RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH

                  APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV

                  FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE

                  RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG

                  QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG

                   AGQRADR"

ORIGIN    

     1 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt

    61 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt

   121 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc

   181 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca

   241 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca

   301 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc

   361 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca

   421 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta

   481 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt

   541 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg

   601 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga

   661 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac

   721 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc

   781 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc

   841 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca

   901 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt

   961 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc

  1021 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg

  1081 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat

  1141 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt

  1201 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa

  1261 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg

  1321 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac

  1381 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg

  1441 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca

  1501 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag

  1561 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc

  1621 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt

  1681 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca

  1741 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa

  1801 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg

  1861 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag

  1921 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg

  1981 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag

   2041agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg

  2101 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc

  2161 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc

  2221 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg

  2281 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca

  2341 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag

  2401 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg

  2461 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca

  2521 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc

  2581 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg

  2641 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat

  2701 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac

  2761 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca

  2821 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc

  2881 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa

  2941 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag

  3001 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca

  3061 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt

  3121 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca

  3181 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg

  3241 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg

  3301 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc

  3361 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga

  3421 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag

  3481 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta

  3541 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc

  3601 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc

   3661 ctatcgcggc cgctggccgc tcaaaaatggctggcctacg gccaggcaat ctaccagggc

  3721 gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg aggtctgcct cgtgaagaag

  3781 gtgttgctga ctcataccag ggcaatggca acaacgttgc gcaaactatt aactggcgaa

  3841 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca

  3901 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc

  3961 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt

  4021 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc

  4081 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat

  4141 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt

  4201 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac

  4261 cccttaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa

  4321 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg

  4381 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct

  4441 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag

  4501 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca

  4561 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc

  4621 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg

  4681 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt

  4741 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct

  4801 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt

  4861 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg

  4921 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta

  4981 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca

  5041 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc

  5101 tgcctcgtga agaaggtgtt gctgactcat accaggctag tccgaggagc tttatgcttg

  5161 taaaccgttt tgtgaaaaaa tttttaaaat aaaaaagggg acctctaggg tccccaatta

  5221 attagtaata taatctatta aaggtcattc aaaaggtcat ccaccggatc aattcccctg

  5281 ctcgcgcagg ctgggtgcca agctctcggg taacatcaag gcccgatcct tggagccctt

  5341 gccctcccgc acgatgatcg tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa

  5401 accctcactg atccgctagt ccgaggcctc gagatctatc gatgcatgcc atggtacccg

  5461 ggagctcgaa ttctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacattata

  5521 cgagccgatg attaattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg

  5581 tcggcgcaaa aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt

  5641 gatttacgac ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt

  5701 ggtgcaccgt gcagtcgata agcccggatc agcttgcaat tcgcgcgcga aggcgaagcg

  5761 gcatgcattt acgttgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag

  5821 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc

   5881gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac

  5941 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc

  6001 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc

  6061 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa

  6121 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg

  6181 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat

  6241 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat

  6301 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg

  6361 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta

  6421 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa

  6481 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc

  6541 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg

  6601 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg

  6661 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc

  6721 aaacaggatt ttcgcctgct ggggcaaacc acgtggaccg cttgctgcaa ctctctcagg

  6781 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc

  6841 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg

  6901 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag

  6961 cgcgaattgc aagctggccg acgcgctggg ctacgtcttg ctggcgttcg gatccgatat

  7021 cgccgtggcg gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac

  7081 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg

  7141 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc

  7201 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat

  7261 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac

  7321 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt

  7381 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa

  7441 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc

   7501 cctcactttc tggctggatg atggggcgattcaggcctgg tatgagtcag caacaccttc

  7561 ttcacgaggc agacctcagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc

  7621 ccacgagcgg acgccgcagc aggagcgcca gaaggccgcc agagaggccg agcgcggccg

  7681 tgaggcttgg acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct

  7741 gacgcggtgg aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg

  7801 cgctccggca gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca

  7861 acgagcctcc ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg

  7921 tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc

  7981 tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg

  8041 gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa

  8101 cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt

  8161 cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg

  8221 cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta

  8281 tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag

  8341 tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga

  8401 ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc

  8461 aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag

  8521 tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga

  8581 aacccaacat acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg

  8641 gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca

  8701 ccgcccacta tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg

  8761 tgctgggcgc gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg

  8821 gcgccactgt cgactacgcc atcatggcga cagcgccttt cctttgggtt ctctatatcg

  8881 ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg

  8941 atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt

  9001 tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc

  9061 cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc

  9121 cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt

  9181 cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca

  9241 tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc

  9301 actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg

  9361 cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc

  9421 tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga

  9481 tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag

  9541 caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg

  9601 cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt

  9661 ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc

   9721tgccggcgct gcgtcgcggg ctttggagcg gcgcagggca acgagccgat cgctgatcgt

  9781 ggaaacgata ggccgctcta gaactagcta gaacta

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