| 出品公司: | Agilent |
|---|---|
| 载体名称: | pShuttle-IRES-hrGFP-2 |
| 质粒类型: | 腺病毒表达载体，哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8.9 kb |
| 5' 测序引物及序列: | pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC |
| 3' 测序引物及序列: | pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG |
| 载体标签: | -- |
| 载体抗性: | 卡那 |
| 备注: | 载体内包含有IRES序列和hrGFP序列。 |
| 产品目录号: | 240082 |
| 稳定性: | 瞬时表达 |
| 组成型: | -- |
| 病毒/非病毒: | 腺病毒 |
pShuttle-IRES-hrGFP-2腺病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格：¥1920
- 货 号：BiovectorShuttle-IRES-hrGFP-2
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).
载体序列
载体质粒图谱pdf版和相关资料下载
载体应用举例
- 公告/新闻
