pLCNICK plasmid干酪乳杆菌基因编辑质粒CRISPR-Cas9载体-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Genome editing for Lactobacillus casei Lc2W

Vector backbone pCas

Backbone size w/o insert (bp)4500

Total vector size (bp)14300

Vector type CRISPR

GROWTH IN BACTERIA

Bacterial Resistance(s)Kanamycin

Growth Temperature30°C

Growth Strain(s)DH5alpha

Copy numberLow Copy

GENE/INSERT 1

Gene/Insert nameCas9 nickase

SpeciesS. pyogenes

Insert Size (bp)4107

MutationD10A

GenBank IDAE004092

GENE/INSERT 2

Gene/Insert namesgRNA

Insert Size (bp)103

GENE/INSERT 3

Gene/Insert namehomology arms of LC2W_2179

Insert Size (bp)2007

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

http://www.biovector.net