pSET4s温度敏感型自杀质粒载体Thermosensitive suicide vector -BioVector NTCC保藏中心

Thermosensitive suicide vector pSET4s

Sequence:

LOCUS       AB055650                4506 bp    DNA     circular SYN 21-MAY-2003
DEFINITION  Thermosensitive suicide vector pSET4s DNA, complete sequence.
ACCESSION   AB055650
VERSION     AB055650.1  GI:15553030
KEYWORDS    .
SOURCE      Thermosensitive suicide vector pSET4s
  ORGANISM  Thermosensitive suicide vector pSET4s
            other sequences; artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Takamatsu,D., Osaki,M. and Sekizaki,T.
  TITLE     Thermosensitive suicide vectors for gene replacement in
            Streptococcus suis
  JOURNAL   Plasmid 46 (2), 140-148 (2001)
   PUBMED   11591139
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Takamatsu,D., Osaki,M. and Sekizaki,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2001) Daisuke Takamatsu, National Institute of
            Animal Health, Laboratory of Molecular Bacteriology; Kannondai
            3-1-1, Tsukuba, Ibaraki 305-0856, Japan
            (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743,
            Fax:81-298-38-7743)
FEATURES             Location/Qualifiers
     source          1..4506                     /organism="Thermosensitive suicide vector pSET4s"                     /mol_type="other DNA"                     /db_xref="taxon:152114"gene            528..689                     /gene="orfC"CDS             528..689                     /gene="orfC"                     /codon_start=1                     /transl_table=11                     /product="ORFC"                     /protein_id="BAB64880.1"                     /db_xref="GI:15553031"                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEY                     ARKESEQKK"gene            756..1454                     /gene="repAts"CDS             756..1454                     /gene="repAts"                     /codon_start=1                     /transl_table=11                     /product="RepAts"                     /protein_id="BAB64881.1"                     /db_xref="GI:15553032"                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMD                     EKKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEI                     LDYIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLL                     DIVDDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRA                     SYAKVLDAETGEIK"gene            complement(1803..2126)                     /gene="lacZ'"CDS             complement(1803..2126)                     /gene="lacZ'"                     /codon_start=1                     /transl_table=11                     /product="LacZ'"                     /protein_id="BAB64882.1"                     /db_xref="GI:15553033"                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTI                     CSDAA"gene            3637..4404                     /gene="spc"CDS             3637..4404                     /gene="spc"                     /codon_start=1                     /transl_table=11                     /product="spectinomycin adenyltransferase"                     /protein_id="BAB64883.1"                     /db_xref="GI:15553034"                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPN                     SDLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPP                     KQEFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFS                     DVRRAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEH                     RERILLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"ORIGIN      
        1 actagttatc ggcataatcg ttaaaacagg cgttatcgta gcgtaaaagc ccttgagcgt
       61 agcgtggctt tgcagcgaag atgttgtctg ttagattatg aaagccgatg actgaatgaa
      121 ataataagcg cagcgccctt ctatttcggt tggaggaggc tcaagggagt atgagggaat
      181 gaaattccct catgggtttg attttaaaaa ttgcttgcaa ttttgccgag cggtagcgct
      241 ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg gggggaaagg aagcgaattt
      301 tgcttccgta ctacgacccc ccattaagtg ccgagtgcca atttttgtgc caaaaacgct
      361 ctatcccaac tggctcaagg gtttaagggg tttttcaatc gccaacgaat cgccaacgtt
      421 ttcgccaacg ttttttataa atctatattt aagtagcttt attgttgttt ttatgattac
      481 aaagtgatac actaacttta taaaattatt tgattggagt tttttaaatg gtgatttcag
      541 aatcgaaaaa aagagttatg atttctctga caaaagagca agataaaaaa ttaacagata
      601 tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc gttagctata gaagaatatg
      661 caagaaagga atcagaacaa aaaaaataag cgaaagctcg cgtttttaga aggatacgag
      721 ttttcgctac ttgtttttga taaggtaatt atatcatggc tattaaaaat actaaagcta
      781 gaaattttgg atttttatta tatcctgact caattcctaa tgattggaaa gaaaaattag
      841 agagtttggg cgtatctatg gctgtcagtc ctttacacga tatggacgaa aaaaaagata
      901 aagatacatg gaataatagt aatattatac aaaatggaaa gcactataaa aaaccacact
      961 atcacgttat atatattgca cgaaatcctg taacaataga aagcgttagg aacaagatta
     1021 agcgaaaatt ggggaatagt tcagttgctc atgttgagat acttgattat atcaaaggtt
     1081 catatgaata tttgactcat gaatcaaagg acgctattgc taagaataaa catatatacg
     1141 acaaaaaaga tattttgaac attaatgatt ttgatattga ccgctatata acacttgatg
     1201 aaagccaaaa aagagaattg aagaatttac ttttagatat agtggatgac tataatttgg
     1261 taaatacaaa agatttaatg gcttttattc gccttagggg agcggagttt ggaattttaa
     1321 atacgaatga tgtaaaagat attgtttcaa caaactctag cgcctttaga ttatggtttg
     1381 agggcaatta tcagtgtgga tatagagcaa gttatgcaaa ggttcttgat gctgaaacgg
     1441 gggaaataaa atgacaaaca aagaaaaaga gttatttgct gaaaatgagg aattaaaaaa
     1501 agaaattaag gacttaaaag agcgtattga aagatacaga gaaatggaag ttgaattaag
     1561 tacaacaata gatttattga gaggagggat tattgaataa ataaaagccc cctgacgaaa
     1621 gtcgaagggg gtttttattt tggtttgatg ttgcgattaa tagatctcgg tgatgacggt
     1681 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     1741 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     1801 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     1861 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     1921 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     1981 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2041 acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
     2101 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2161 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2221 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2281 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2701 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2941 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3361 cgtgtagata actagtgttc gtgaatacat gttataataa ctataactaa taacgtaacg
     3421 tgactggcaa gagatatttt taaaacaatg aataggttta cacttacttt agttttatgg
     3481 aaatgaaaga tcatatcata tataatctag aataaaatta actaaaataa ttattatcta
     3541 gataaaaaat ttagaagcca atgaaatcta taaataaact aaattaagtt tatttaatta
     3601 acaactatgg atataaaata ggtactaatc aaaatagtga ggaggatata tttgaataca
     3661 tacgaacaaa ttaataaagt gaaaaaaata cttcggaaac atttaaaaaa taaccttatt
     3721 ggtacttaca tgtttggatc aggagttgag agtggactaa aaccaaatag tgatcttgac
     3781 tttttagtcg tcgtatctga accattgaca gatcaaagta aagaaatact tatacaaaaa
     3841 attagaccta tttcaaaaaa aataggagat aaaagcaact tacgatatat tgaattaaca
     3901 attattattc agcaagaaat ggtaccgtgg aatcatcctc ccaaacaaga atttatttat
     3961 ggagaatggt tacaagagct ttatgaacaa ggatacattc ctcagaagga attaaattca
     4021 gatttaacca taatgcttta ccaagcaaaa cgaaaaaata aaagaatata cggaaattat
     4081 gacttagagg aattactacc tgatattcca ttttctgatg tgagaagagc cattatggat
     4141 tcgtcagagg aattaataga taattatcag gatgatgaaa ccaactctat attaacttta
     4201 tgccgtatga ttttaactat ggacacgggt aaaatcatac caaaagatat tgcgggaaat
     4261 gcagtggctg aatcttctcc attagaacat agggagagaa ttttgttagc agttcgtagt
     4321 tatcttggag agaatattga atggactaat gaaaatgtaa atttaactat aaactattta
     4381 aataacagat taaaaaaatt ataaaaaaat tgaaaaaatg gtggaaacac ttttttcaat
     4441 ttttttgttt tattatttaa tatttgggaa atattcattc taattggtaa tcagatttta
     4501 gaaaac

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