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FLO-1 cell line人食管腺癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥99865
  • 货  号:FLO-1
  • 产  地:北京
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FLO-1 cell line人食管腺癌细胞株

Keywords: Distal oesophageal adenocarcinoma
Cell Line Description: FLO-1 was established from a primary distal oesophageal adenocarcinoma in a 68 year-old Caucasian male in 1991. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer

Species: Human
Tissue of Origin: Oesophagus
CellType: Epithelial
Growth Mode: Adherent
DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 12,14
D7S820: 8
THO1: 6
TPOX: 9,11
vWA: 16
Subculture Routine: Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 105 cells/cm2.
Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

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