pREP4xN plasmid裂殖酵母表达载体Gateway质粒-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.

Vector type Yeast Expression

Selectable markers URA4+

Bacterial Resistance(s) Chloramphenicol and Ampicillin

Growth Temperature 37°C

Growth Strain(s) ccdB Survival

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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