pASK-IBA37plus plasmid vector大肠杆菌四环素诱导表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pASK-IBA37plus plasmid vector大肠杆菌四环素诱导表达质粒载体

Description Expression plasmid. The expression cassette is under transcriptional control of
the tetracycline promoter/operator. The expressed recombinant protein will be
localized in the cytoplasm. Affinity tag 6xHistidine-tag for the purification of recombinant protein. The affinity tag is
fused to the N-terminus of the recombinant protein and can be removed by
cleavage with factor Xa. Bacterial Expression Expression is induced upon addition of 200 µg anhydrotetracycline (order no.:
2-0401-001; 2-0401-002) per 1 liter E. coli shaking culture (A550 = 0.5). Expression strain Any E. coli strain. The tet-promoter works independently from the genetic
background of E. coli. Resistance Ampicillin


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