pDOE-10载体质粒-BioVector NTCC典型培养物保藏中心

pDOE-10 vector. [Description]: pDOE-10 is a single-vector self-contained BiFC construct based on the mVenus210 fragment system, and the specific fusion orientation for this construct is X::NmVenus210--CVenus210::X. The linker for the X::CVenus210 fragment contains a StrepII-FLAG-StrepII linker for detection or pull-downs. The dual ORF expresssion cassettes are driven by identical Ubiquitin10 promotors with fused Omega viral translation enhancers. The pDOE-10 construct also features an independent integrated cassette that expresses the XT-Golgi-mTq2 marker from the MAS 2' promotor to specifically label the medial Golgi cisternae with mTurquoise2. Bacterial selection: Kanamycin (50 ug/mL).  [Stock No.]: CD3-1910