Ba/F3 EGFR D770-N771ins_SVD Cell Line稳转细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Ba/F3 EGFR D770-N771ins_SVD Cell Line稳转细胞株

Cell line name: Ba/F3 EGFR D770-N771insSVD Cell Line
Gene ID/Accession #: NM_005228.3
Host cell line Mouse Ba/F3 cell line
Cell type: Pro-B cells
Description: Stable Ba/F3 clone expressing exogenous EGFR gene bearing exon20 D770-N771 SVD
insertion.
Quantity: One vial of frozen cells (5X106 per vial)
Stability: Stable in culture over a minimum of 10 passages
Application: Drug screening and biological assays
Freeze medium: 70% RMPI-1640 + 20% FBS + 10% DMSO
Propagation medium: RPMI 1640 + 10% FBS
Selection marker: Puromycin
Morphology: Mostly single, round (some polymorph) cells in suspension
Subculture: Split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105 cells/ml
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: Approximately 20 hours
Mycoplasma status: Negative
Biosafety level: 1


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