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AK-D cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥79865
  • 货  号:AK-D BEC
  • 产  地:北京
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AK-D cell line细胞株

Cell Line Name: AK-D
Other Collection No.:  CCL 150

Keywords: Foetal cat lung normal
Cell Line Description: A clonal derivative of mass cultures of foetal feline lung after 14 passages in vitro. Susceptible to panleukopenia, feline herpes and feline picornaviruses.
Species: Feline
Tissue of Origin: lung
CellType: Epithelial
Growth Mode: Adherent
Karyotype: Diploid

Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm² using 0.05% trypsin and 0.03% EDTA; 5% CO2; 37°C.
Culture Medium: Ham's F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

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