MC57 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

MC57 cell line细胞株

Product category
Animal cells
Organism
Mus musculus, mouse
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Disease
Fibrosarcoma
Applications
3D cell culture
Specific applications
The cell line has been used extensively since 1976 as an H-2b cell line for cell-mediated cytotoxicity assays; the cells express both H-2Kb and H-2Db.
This cell line is the laboratory standard in many laboratories for mouse viral immunity studies.
Growth properties
Adherent
Derivation
The MC57 is a fibrosarcoma cell line established from a tumor arising in a C57BL/6 mouse after treatment with methylcholanthrene.
Strain
C57BL/6J
Immortalization method
Methylcholanthrene induced
Comments
The cells express both H-2Kb and H-2Db.
MC57 is infectible by viruses.
Complete medium
The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature
37°C
Atmosphere
95% Air, 5% CO2

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