首页 » pGEM-7Zf(–)体外转录载体环状ssDNA生产质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pGEM-7Zf(–)体外转录载体环状ssDNA生产质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥19865
  • 货  号:pGEM-7Zf(–)
  • 产  地:北京
点击询问我要采购
 竭诚为您服务!
BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

电话:+86-010-53513060. 工作QQ:1843439339 (微信同号)

邮件:Biovector@163.com

手机:18901268599

地址:北京

已注册
 

pGEM-7Zf(–)体外转录载体环状ssDNA生产质粒

The pGEM-7Zf(+) and pGEM-7Zf(–) Vectors are derivatives of the pGEM-3Zf(+) Vector and contain the origin of replication of the filamentous phage f1. These plasmids serve as standard cloning vectors, as templates for in vitro transcription and can be used for the production of circular ssDNA. These plasmids contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base™ System. pGEM-7Zf(+) and pGEM-7Zf(–) Vectors are identical except for the orientation of the f1 origin.


Features - Benefits
Blue/White Screening: Allows the easy identification of recombinant clones.
Versatile: These standard cloning vectors are equipped for single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
Convenient: Multiple cloning site provides a selection of restriction sites for cloning.
Unidirectional Deletions: Restriction sites are positioned conveniently for use with the Erase-a-Base™ System.

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net

您正在向 biovector.net  发送关于产品 pGEM-7Zf(–)体外转录载体环状ssDNA生产质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问

点击“立即发送”后,我们将在1个工作日内与您取得联系。