LIM 1215 cell line细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
- 价 格:¥79865
- 货 号:BioVector-CBA-0161
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
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地址:北京
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Description: LIM1215 is a human colorectal carcinoma cell line. It was derived from a biopsy of involved omentum, with the primary lesion found in the ascending colon. The patient, a 34 year old male, had a family history of colorectal carcinoma. Description Key Words: Human colorectal carcinoma Also Known As: No other names Organism: Human (Homo sapiens) Strain: N/A Tissue: Omental metastasis Growth Properties: LIM1215 is loosely adherent and grows in islands which may pile up. Morphology: Epithelial Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% Foetal Calf Serum + Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM. Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. Recovery from thaw may take 3 days. Subculturing Procedure: Medium Renewal: 2-3 times per week. Do not allow the cells to exceed 60-70% confluent. At higher density cells will become less adherent. Subcultivation Ratio: 1:4 – 1:8, Seeding density 1.2 x104cells/cm2. Split subconfluent cultures. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. Culture Conditions: Incubate the culture at 37°C with 5% CO2. Cryoprotectant Medium: 10% DMSO + 90% FCS Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability. Additional Information: Colorectal cancer from HPNCC patient, mutated beta-catenin, secrete TGF-alpha and are responsive to TGFalpha and EGF, A33 positive, MSI, widely used in scientific literature. Medium:Growth Condition:BioVector NTCC Inc.www.biovector.net
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