LIM 2550 cell line细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
- 价 格:¥79865
- 货 号:BioVector-CBA-0169
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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Description: Colon carcinoma Description Key Words: Human colon carcinoma Also Known As: No other names Organism: Human (Homo sapiens) Strain: N/A Tissue: Colon Growth Properties: Cell line grows as floating and loose aggregates with some adherent cells. Morphology: Epithelial Growth Medium: RPMI1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% Foetal Calf Serum (FCS) + Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10μM. Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. Subculturing Procedure: Medium Renewal: 3 times each week. Feed and passage as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding). Subcultivation Ratio: 1:4 – 1:8, Seeding density 1 - 2 x104 cells/cm2. Split sub-confluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. Cells may take 2-3 days to seed after thawing or trypsinisation. Culture Conditions: Cells maintained at 37°C and 5% CO2. Cryoprotectant Medium: 10% DMSO + 90% FCS Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability. Additional Information: Derived from colon carcinoma, adherent and loose aggregatesMSI, A33 negative, truncated APC (stop at aa 1544)Mutated p53 (aa G245D)Heterozygous mutated K-Ras (aa Q61H)Mutated PI3K (aa H1047R p110alpha).Medium:Growth Condition:BioVector NTCC Inc.www.biovector.net
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