NZM40 cell line细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
- 价 格:¥79865
- 货 号:BioVector-CBA-1381
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
Description: NZM40 is an adherent cell line derived from a metastatic melanoma from a 47yr old female patient, in 2004. Description Key Words: Human metastatic melanoma Also Known As: No other names Organism: Human (Homo sapiens) Growth Properties: Adherent. Cells are large and the yield per flask is low at around 2 million cells per T75 flask. Doubling Time: 38 hours Morphology: Epithelial Growth Medium: This cell line was established by the depositor in: Alpha modified Minimum Essential Medium (MEM) supplemented with 5% Foetal Bovine Serum (FBS), 5 ug/ml Insulin, 5 ug/ml Transferrin, 5 ng/ml Sodium Selenite (ITS), in an atmosphere of 5%O2, 5%CO2 and 90%N2. At CellBank Australia it has been cultured in: Alpha modified Minimum Essential Medium (MEM) supplemented with 10% Foetal Bovine Serum (FBS), 5 ug/ml Insulin, 5 ug/ml Transferrin, 5 ng/ml Sodium Selenite (ITS), in an atmosphere of 5%CO2. Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. Subculturing Procedure: Medium Renewal: 3 times per week. Subcultivation Ratio: 1:4 - 1:8, Seeding density 0.7 x104cells/cm2. Split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. Note that this cell line is trypsin sensitive so care should be taken to minimise exposure time to trypsin. Culture Conditions: Incubate the culture at 37°C with 5% CO2. Cryoprotectant Medium: 10% DMSO + 90% FCS Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability. Additional Information: Aneuploid 1.82x and 3.06x diploid;Mutations: p53 mutant (del 249-253), NRAS mutant Q61H, PTEN, BRAFWild-type for CDKN2A/B, PIK3CATemozolomide resistant; MGMT not expressed. Medium:Growth Condition:BioVector NTCC Inc.www.biovector.net
您正在向 biovector.net 发送关于产品 NZM40 cell line细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 的询问
- 公告/新闻