NZM5 cell line细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
- 价 格:¥79865
- 货 号:BioVector-CBA-1388
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
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地址:北京
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Description: NZM5 is a semi-adherent cell line derived from a 79-year-old male patient with metastatic melanoma (axillary lymph node). Description Key Words: Human metastatic melanoma Also Known As: No other names Organism: Human (Homo sapiens) Tissue: Melanoma metastatic to axillary lymph node Growth Properties: Semi-Adherent, the cells grow as a loosely adherent monolayer and also in suspension Doubling Time: 48 hours Morphology: Epithelial and rounded in suspension Growth Medium: This cell line was established by the depositor in: Alpha modified Minimum Essential Medium (MEM) supplemented with 5% Foetal Bovine Serum (FBS), 5 ug/ml Insulin, 5 ug/ml Transferrin, 5 ng/ml Sodium Selenite (ITS), in an atmosphere of 5%O2, 5%CO2 and 90%N2. At CellBank Australia it has been cultured in: Alpha modified Minimum Essential Medium (MEM) supplemented with 10% Foetal Bovine Serum (FBS), 5 ug/ml Insulin, 5 ug/ml Transferrin, 5 ng/ml Sodium Selenite (ITS), in an atmosphere of 5%CO2. Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. Subculturing Procedure: Medium Renewal: 2-3 times per week. Subcultivation Ratio: 1:5, Seeding density 4.0 x104cells/cm2. Split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 2 minutes. Cells may take 2-3 days to seed after thaw. They are trypsin sensitive, so care should be taken to minimise exposure time to trypsin. Culture Conditions: Incubate the culture at 37°C with 5% CO2. Cryoprotectant Medium: 10% DMSO + 90% FCS Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability. Additional Information: Wild-type for TP53, PIK3CA, CDKN2A/B, PTEN, NRAS, BRAFClose to diploid: DNA content 40-43 chromosomes Resistant to temozolomide MGMT expressed.Medium:Growth Condition:BioVector NTCC Inc.www.biovector.net
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