NZM7 cell line细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Description: NZM7 is an adherent metastatic melanoma cell line derived from the cervical lymph node of a 36 year old male patient.



Description Key Words: Human metastatic melanoma from cervical lymph node



Also Known As: No other names



Organism: Human (Homo sapiens)



Tissue: Melanoma metastatic from cervical lymph node



Growth Properties: Adherent



Doubling Time: 49 hours



Morphology: Heterogeneous morphology, with elongated cells



Growth Medium: This cell line was established by the depositor in: Alpha modified Minimum Essential Medium (MEM) supplemented with 5% Foetal Bovine Serum (FBS), 5 ug/ml Insulin, 5 ug/ml Transferrin, 5 ng/ml Sodium Selenite (ITS), in an atmosphere of 5%O2, 5%CO2 and 90%N2.



At CellBank Australia it has been cultured in: Alpha modified Minimum Essential Medium (MEM) supplemented with 10% Foetal Bovine Serum (FBS), 5 ug/ml Insulin, 5 ug/ml Transferrin, 5 ng/ml Sodium Selenite (ITS), in an atmosphere of 5%CO2.



Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.



Subculturing Procedure:



Medium Renewal: 2-3 times per week.



Subcultivation Ratio: 1:4 - 1:8, Seeding density 1.3 x104cells/cm2. Split subconfluent cultures(70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.



Culture Conditions: Incubate the culture at 37°C with 5% CO2.



Cryoprotectant Medium: 10% DMSO + 90% FCS



Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.



Additional Information:

_ Aneuploid 2.18x diploid 55-59 chromosomes per cell

_ Mutations:

Wild-type for CDKN2A/B, PIK3CA, NRAS
BRAF V600E mutation
Heterogeneous for p53 mutation: some cells with p53 mutation 241S/P
_ Resistant to temozolomide

_ MGMT expressed

Medium:
Growth Condition:

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