SIRMu-1 cell line细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
- 价 格:¥79865
- 货 号:BioVector-CBA-1665
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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Description: SIRMu-1 is an immortalized rat Müller-1 cell line, monoclonal, derived from 2 sequential single cell/clonal isolations.Note: Müller cells are a major type of glial cells in the retina of the eye. Organism: Rat (Rattus norvegicus) Strain: Sprague-Dawley Tissue: Retina Growth Properties: Adherent Morphology: Epithelial-like Growth Medium: EMEM + 20% Foetal Calf Serum (FCS) + 25mM Glucose + 2mM L-Glutamine Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. NOTE: This cell line may take up to 48hours to seed. Subculturing Procedure: Medium Renewal: 2-3 times per week Subcultivation Ratio: 1:5 - 1:10, split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.At thaw, the cells should be seeded at a density of 2-3 x 104cells/cm2. After recovery from thaw (48-72 hours), they can be seeded at 0.5-1.0 x 104cells/cm2.Flasks will require passaging twice weekly at a 1:10 seeding density. Culture Conditions: Incubate the culture at 37°C with 5% CO2. Cryoprotectant Medium: 10% DMSO + 90% FCS. Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability. Additional Information: The cells express the Müller and/or glial cells markers:Glutamine SynthetaseS-100 ProteinVimentinGlial Fibrillary Acidic Protein (GFAP)Gender: MaleMedium:Growth Condition:BioVector NTCC Inc.www.biovector.net
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