pNIC-CTHF plasmid vector原核表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥49865
- 货 号:pNIC-CTHF
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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pNIC-CTHF plasmid vector原核表达质粒载体
(Empty Backbone) SGC Empty backbone for bacterial expression
pET-28-a
(Search Vector Database)
Backbone manufacturerNovagen
Backbone size (bp)5330
Vector typeBacterial Expression
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin
Growth Temperature37°C
Growth Strain(s)DH5alpha
Growth instructionsFor plasmid propagation and cloning use any E. coli strain not expressing T7 RNA polymerase. For expression, use a strain that expresses T7 RNA polymerase, e.g. BL21(DE3).
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameNone
GenBank IDEF199844
Tag / Fusion Protein
TEV - His6 - Flag (C terminal on backbone)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteLigation-Independent cloning; cut with BfuAI (destroyed during cloning)
3′ cloning siteLigation-Independent cloning; cut with BfuAI (destroyed during cloning)
5′ sequencing primerT7F
3′ sequencing primerT7R
Primers for LIC cloning:
Upstream: add TTAAGAAGGAGATATACTATG to the 5’ end (ATG in-frame with the desired coding
sequence).
Downstream: add GATTGGAAGTAGAGGTTCTCTGC to 5’ end of downstream primer (no termination codon!)
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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