293T/17 [HEK 293T/17] cell line人胚肾细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

293T/17 [HEK 293T/17] cell line人胚肾细胞株

293T/17 [HEK 293T/17]
BioVector-CRL-11268

Product category
Human cells
Organism
Homo sapiens, human
Morphology
epithelial
Tissue
kidney
Applications
3D cell culture
High-throughput screening
Toxicology
Vaccine development

BSL 2

Specific applications
These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.

Growth properties
Adherent
Derivation
The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17.
Age
fetus
Antigen expression
SV40 T antigen
Genes expressed
SV40 T antigen
Comments
293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line. ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line. ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554) amphotropic envelope-expression packaging cell line.

Complete medium
The base medium for this cell line is  Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature
37°C
Atmosphere
95% Air, 5% CO2

STR profiling
Amelogenin: X
CSF1PO: 11, 12
D13S317: 12, 14
D16S539: 9, 13
D5S818: 8, 9
D7S820: 11
THO1: 7, 9.3
TPOX: 11
vWA: 16, 18, 19

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