Streptomyces nodosus ZJB2016050 CCTCC M2017426 strain产两性霉素B的重组结节链霉菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：Streptomyces nodosus ZJB2016050 CCTCC M2017426
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Streptomyces nodosus ZJB2016050 CCTCC M2017426 strain产两性霉素B的重组结节链霉菌菌株
a kind of recombination streptomyces nodocus for producing amphotericin B and its applications, it is described recombination streptomyces nodocus be knock out streptomyces nodocus ZJB2016050 (Streptomyces nodosus ZJB2016050) interior amphotericin B competition branch after, then by functional gene tandem sequence shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 import obtain；By import feature gene, amphotericin B skeleton is made to synthesize precursor supply amount General Promotion, improves strain to the utilization rate of nutriment, change the nutriment flow direction of target product.AmB yield about 20% is improved by knocking out competition branch, by-product amphotericin A reduces by 40%, three classes precursor acetyl coenzyme A is improved by being overexpressed the verifying of concatenation function gene, the yield of amphotericin B is substantially improved in malonyl coenzyme A and methylmalonyl CoA, finally in 5L ferment tank, amphotericin B yield can reach 16g/L.一种产两性霉素B的重组结节链霉菌（ Streptomyces nodosus ） ，由编号为CCTCC NO：M 2017426的结节链霉菌ZJB2016050基因敲除PKS5基因簇获得。
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
a kind of recombination streptomyces nodocus for producing amphotericin B and its applications, it is described recombination streptomyces nodocus be knock out streptomyces nodocus ZJB2016050 (Streptomyces nodosus ZJB2016050) interior amphotericin B competition branch after, then by functional gene tandem sequence shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 import obtain；By import feature gene, amphotericin B skeleton is made to synthesize precursor supply amount General Promotion, improves strain to the utilization rate of nutriment, change the nutriment flow direction of target product.AmB yield about 20% is improved by knocking out competition branch, by-product amphotericin A reduces by 40%, three classes precursor acetyl coenzyme A is improved by being overexpressed the verifying of concatenation function gene, the yield of amphotericin B is substantially improved in malonyl coenzyme A and methylmalonyl CoA, finally in 5L ferment tank, amphotericin B yield can reach 16g/L.一种产两性霉素B的重组结节链霉菌（ Streptomyces nodosus ） ，由编号为CCTCC NO：M 2017426的结节链霉菌ZJB2016050基因敲除PKS5基因簇获得。
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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