Caco-2 BBE Cell Line人结肠癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥798685
- 货 号：Caco-2 BBE
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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Caco-2 BBE Cell Line人结肠癌细胞株
Caco-2 BBE Cell LineCat No.: NTCC932513Product categoryHuman cellsOrganismHomo sapiens, humanCell typeenterocyteMorphologyepithelialTissueLarge intestine; ColonDiseaseAdenocarcinoma; ColorectalApplications3D cell cultureCancer researchHigh-throughput screeningToxicologyCharacteristicsGrowth propertiesAdherentDerivationThe Caco-2 BBE (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line by limiting dilution.The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.Age72 yearsEthnicityWhiteGenderMaleExpression markersEpidermal growth factor (EGF)CommentsThe clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.Caco-2 BBE cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon.Isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II.The cells express substantial levels of BB myosin I similar to that of the human enterocyte.Although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.Complete mediumThe base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml human transferrin; fetal bovine serum to a final concentration of 10%.Temperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.1.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).2.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.3.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to7 minutes.4.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). pH (7.0 to 7.6).5.Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet. Subculturing procedureVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.1.Remove and discard culture medium.2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.5.Add appropriate aliquots of the cell suspension to new culture vessels.6.Incubate cultures at 37°C.Subculture at 85% of confluence (about every 5 to 7 days).Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommendedMedium Renewal: Twice per weekReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSOMycoplasma contaminationNot detected
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Caco-2 BBE Cell LineCat No.: NTCC932513Product categoryHuman cellsOrganismHomo sapiens, humanCell typeenterocyteMorphologyepithelialTissueLarge intestine; ColonDiseaseAdenocarcinoma; ColorectalApplications3D cell cultureCancer researchHigh-throughput screeningToxicologyCharacteristicsGrowth propertiesAdherentDerivationThe Caco-2 BBE (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line by limiting dilution.The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.Age72 yearsEthnicityWhiteGenderMaleExpression markersEpidermal growth factor (EGF)CommentsThe clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.Caco-2 BBE cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon.Isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II.The cells express substantial levels of BB myosin I similar to that of the human enterocyte.Although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.Complete mediumThe base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml human transferrin; fetal bovine serum to a final concentration of 10%.Temperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.1.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).2.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.3.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to7 minutes.4.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). pH (7.0 to 7.6).5.Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet. Subculturing procedureVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.1.Remove and discard culture medium.2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.5.Add appropriate aliquots of the cell suspension to new culture vessels.6.Incubate cultures at 37°C.Subculture at 85% of confluence (about every 5 to 7 days).Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommendedMedium Renewal: Twice per weekReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSOMycoplasma contaminationNot detected
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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