pEF1s-hIgG1CH人hIgG1重链恒定区抗体分泌型表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pEF1s-hIgG1CH人hIgG1重链恒定区抗体分泌型表达质粒载体

pEF1-hIgG1CH is a cloning plasmid that expresses the constant region of the human IgG1 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pEF1-hIgG1CL.
pEF1s-hIgG1CH additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Key features of pEF1-hIgG1CH

Encodes the human IgG1 CH constant region
Long-lasting expression due to the composite hEF1-HTLV promoter
Selectable with Zeocin™ in both bacterial and mammalian cells
Available with or without the IL-2 signal sequence



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