pCAS plasmid vector大肠杆菌基因编辑Red重组CRISPR-Cas9质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pCAS plasmid vector大肠杆菌基因编辑Red重组CRISPR-Cas9质粒

PURPOSE
Constitutive expression of cas9 and inducible expression of lambda RED and sgR
Vector backbone pKD46
Backbone size w/o insert (bp)6300
Modifications to backboneReplace Amp with Kan
Vector typeBacterial Expression, CRISPR ; Lambda Red recombinase

GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin
Growth Temperature30°C
Growth Strain(s)DH5alpha
Growth instructionsMust be grown at 30C! Vector has temperature-sensitive replication (RepA101ts)
Copy numberLow Copy

GENE/INSERT
Gene/Insert namecas9
SpeciesS. pyogenes MGAS5005
Insert Size (bp)4500
Promoternative cas9 promoter

CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteunknown (unknown if destroyed)
3′ cloning siteunknown (unknown if destroyed)
5′ sequencing primerpENTR1A-3 (GTAACATCAGAGATTTTGAGACAC)
3′ sequencing primerAraC-F-New (GATGTAGCCGTCAAGTTGTCA)

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net