pADL-23C plasmid vector噬菌体展示系统质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥989865
- 货 号：pADL-23C
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pADL-23C plasmid vector噬菌体展示系统质粒载体
The pADL™-23c phagemid is a type 3+3 phage display vector with a cloning site for display on the N-terminal side of the full length gene III protein. Secretion in the periplasm of the fusion protein is driven by the PelB leader peptide. A HIS tag and a Myc tag followed by an amber codon are conveniently located before the gene III protein. Phage display will be done using bacterial strains that suppress the amber codon while growth on non-suppressive strains will result in the expression of free scFvs or Fab fragments in the periplasm space; a classical application of this vector is the production of free scFv and detection of its binding through the Myc tag.The pADL™-2x phagemid vector series offers optimal characteristics for phage display with optimized expression of the fusion protein for strong display, suppressible amber codon to direct the fusion either as a gene III fusion or as a secretedfree entity, cloning site amenable to multiple cloning strategies and varied linker and display options. The fusion protein is under the control of the lac promoter, allowing metabolic repression by glucose and induction by IPTG. A copy of the lambda t1 terminator located downstream gene III prevents leakiness of the transcription during induction, in particular preventing excessive expression of the beta-lactamase and rapid consumption of ampicillin.The vector contains two origins of replication, the f1 origin, which packages the single-stranded phagemid DNA into nascent virions, and the pMB1 origin of replication derived from pBR322, which results in a high-copy-number phagemid; the pMB1 sequence lacks the rop gene and carries a point mutation in the RNAII transcript (G 2975 in pBR322 to T 1304 on the reverse complement strand; Lin-Chao 1992)
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The pADL™-23c phagemid is a type 3+3 phage display vector with a cloning site for display on the N-terminal side of the full length gene III protein. Secretion in the periplasm of the fusion protein is driven by the PelB leader peptide. A HIS tag and a Myc tag followed by an amber codon are conveniently located before the gene III protein. Phage display will be done using bacterial strains that suppress the amber codon while growth on non-suppressive strains will result in the expression of free scFvs or Fab fragments in the periplasm space; a classical application of this vector is the production of free scFv and detection of its binding through the Myc tag.The pADL™-2x phagemid vector series offers optimal characteristics for phage display with optimized expression of the fusion protein for strong display, suppressible amber codon to direct the fusion either as a gene III fusion or as a secretedfree entity, cloning site amenable to multiple cloning strategies and varied linker and display options. The fusion protein is under the control of the lac promoter, allowing metabolic repression by glucose and induction by IPTG. A copy of the lambda t1 terminator located downstream gene III prevents leakiness of the transcription during induction, in particular preventing excessive expression of the beta-lactamase and rapid consumption of ampicillin.The vector contains two origins of replication, the f1 origin, which packages the single-stranded phagemid DNA into nascent virions, and the pMB1 origin of replication derived from pBR322, which results in a high-copy-number phagemid; the pMB1 sequence lacks the rop gene and carries a point mutation in the RNAII transcript (G 2975 in pBR322 to T 1304 on the reverse complement strand; Lin-Chao 1992)
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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