IGR39 cell line人黑色素瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

IGR39 cell line人黑色素瘤细胞株

Cell line:
IGR-39
DSMZ no.:
ACC 239
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the primary (achromic) cutaneous tumor (left thigh) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); same patient as cell line IGR-37 (DSM ACC 237); described as tumorigenic in nude mice
Reference(s):
14363
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
adherent epithelial-like cells growing in monolayers; image; image
Medium:
85% Dulbecco's MEM + 15% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split subconfluent or confluent culture 1:4 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30 hours
Harvest:
maximal density of about 6-8 x 106/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded tetra-/pentaploid karyotype - 104-112<4n>XXY - rearrangements include der(1)t(1;?21)(p13;q11), der(16)t(1;16)(p13;q23) also present in the autologous sister line IGR-37
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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