IGR37 cell line人黑色素瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

IGR37 cell line人黑色素瘤细胞株

Cell line:
IGR-37
DSMZ no.:
ACC 237
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the lymph node metastasis (groin) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); same patient as cell line IGR-39 (DSMZ ACC 239); described as strong tumorigenic in nude mice
Reference(s):
14363
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing as monolayers; image ; image; image
Medium:
85% Dulbecco's MEM + 15% h.i. FBS
Subculture:
seed out at ca. 2.5 x 106 cells/75 cm2; split confluent culture 1:2 every 7-14 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca.48-72 hours
Harvest:
cell harvest of about 2-3 x 105/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 11% polyploidy - 78(67-78)<3n>XXY, +1, +3, +5, +7, +7, +8, +9, -10, -12, -13, -14, -15, -17, +18, +20, +20, +2mar, der(1)t(1;?21)(p13;q11)x2, del(9)(p13p21), der(10)t(9;10)(q11/21;q23/25), i(13q)x1-2, der(16)t(1;16)(p11/13;q21/23)x2, der(19)t(17;19)(q12;p13)x1-2 - rearrangements at 1p12/22, 19p13 and +7 recur in malignant melanoma
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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