RFP‐I‐scel plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥49865
- 货 号:RFP‐I‐scel
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
RFP‐I‐scel plasmid vector质粒载体
The Ligand BindingDomain (LBD) of GR was amplified from pCI-nGFP-C656G GR and cloned into pEGFP-C2 in the SacII and BamHI sites. The C656G mutation of GR that makes it more responsive to ligand andtrasnfers it more quickly to the nucleus. Similarly, the I-SceI cDNA was amplified from HA-I-SceI and cloned into the previously describedGFP-GRLBD into the SacI and SalI restriction sites. Finally, the chimeric I-SceI–GR cDNA was subcloned into pDsRed-Monomer-C1 (Clontech) into the SacI and BamHI sites.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The Ligand BindingDomain (LBD) of GR was amplified from pCI-nGFP-C656G GR and cloned into pEGFP-C2 in the SacII and BamHI sites. The C656G mutation of GR that makes it more responsive to ligand andtrasnfers it more quickly to the nucleus. Similarly, the I-SceI cDNA was amplified from HA-I-SceI and cloned into the previously describedGFP-GRLBD into the SacI and SalI restriction sites. Finally, the chimeric I-SceI–GR cDNA was subcloned into pDsRed-Monomer-C1 (Clontech) into the SacI and BamHI sites.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
- 公告/新闻