PID00003 pmCherry-pur4 Plasmid质粒载体-BioVector NTCC典型培养物保藏中心

Base Pairs 5882
Source Tokuko Haraguchi, Osaka University, Japan
Publications Iwamoto M, Mori C, Hiraoka Y, Haraguchi T. 2014. Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena. Gene 534(2):249-55.
Vector Type Vector for adding mCherry to 3' end of a gene and selecting with puromycin
E. coli selection Ampicillin
Tetrahymena selection Puromycin
Selection cassette pPur4-opt [MTT1(promoter)-pur-opt; inducible promoter]
Expression cassette Allows 3' addition of mCherry to GOI. Targeting determined by selection of 5' and 3' flanks.
Notes 5' targeting sequence, including promoter and gene can be added into MCS 5' of mCherry sequence. 3' targeting flank can be added in a second MCS 3' of the Pur4-opt cassette (see map).
Sequence File pmCherry-pur4.docx
Plasmid Map

Supplier: BioVector NTCC Inc.
www.biovector.net