PID00010 pMTT1GOI-GFPBTU1 (pMTT-NRK2-GFP) Plasmid质粒载体-BioVector NTCC典型培养物保藏中心

Base Pairs 9774
Source Jacek Gaertig lab, University of Georgia
Publications
Vector Type Add 3' GFP to GOI. Targets BTU1 locus
E. coli selection Ampicillin
Tetrahymena selection Taxol - only with strains carrying mutation in Btu1 gene conferring taxol sensitivity
Selection cassette Taxol in strains with BTU1 mutation conferring taxol sensitivity
Expression cassette MTT1 (inducible promoter) driven GOI tagged with GFP, targeted to BTU1 locus
Notes can ONLY be used with CU522 and related strains to target BTU1 locus using taxol selection. Additional selection cassette could be added to target other strains.

1. The sequence of the 5' restriction sites is



2. Grow in a methylation minus Ecoli strain
Sequence File pMTT-NRK2-GFP.docx
Plasmid Map

Supplier: BioVector NTCC Inc.
www.biovector.net